Biolegend, 635402, Purified anti-TIMP-2 Antibody, 100μg

TIMP-2 (Tissue inhibitor of metalloproteinase 2) is a nonglycosylated protein that belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 24 kD. The mechanisms of TIMPs inhibit angiogenesis appear to be specific for each member of the TIMP family. TIMP-2 binds to the surface of human endothelial cells through interaction with the integrin α3β1, and abrogates angiogenic factor induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. Clone F27P3A4 has been shown to be useful for western blotting, immunohistochemistry and ELISA of human, mouse, rabbit, chicken, zebrafish, and pig TIMP-2.
100μg
Verified Reactivity: Human
Reported Reactivity: Mouse, Rabbit, Chicken, Zebrafish, Pig
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ovalbumin-conjugated synthetic Peptide DSGNDIYGNPI
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Murray G et al. 1998. Gut. 43:791. Fromique O, et al. 2008. J. Biol. Chem. 283:30549. PubMed
Product Citations: Hrabia A, et al. 2022. Poult Sci. 101:101915. PubMed Fromigué O, et al. 2008. J Biol Chem. 283:30549. PubMed
RRID: AB_2287490 (BioLegend Cat. No. 635401) AB_2204826 (BioLegend Cat. No. 635402)
Structure: Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain, predicted molecular weight 24 kD.
Distribution: Extracellular
Function: TIMP-2 binds to the surface of human endothelial cells through interaction with the integrin α3β1, and abrogates angiogenic factor induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition.
Interaction: MMP2, MMP8, MMP14, CD49C
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology
Molecular Family: Enzymes and Regulators
Antigen References: 1. Stetler-Stevenson, et al. 1989. J. Biol. Chem. 264:17374
Gene ID: 7077
UniProt: View information about TIMP-2 on UniProt.org
Clone: F27P3A4
Regulatory Status: RUO
Other Names: Metalloproteinase inhibitor 2, Tissue inhibitor of metalloproteinases 2, TIMP2
Isotype: Mouse IgG2a, κ