Biolegend, 635602, Purified anti-ATIC Antibody, 100μg

ATIC (AICAR transformylase/IMP cyclohydrolase) belongs to the purH family, expressed in the cytoplasm, predicted molecular weight approximately 65 kD. ATIC catalyzes the penultimate and final steps in the de novo purine nucleotide biosynthesis pathway. ATIC acts as a bifunctional enzyme catalyze the formation of FAICAR and IMP. AICA-ribosiduria is a recently discovered inherited metabolic disease caused by a defect in final steps of purine de novo biosynthesis. Clone F38P7H9 has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat, frog and fruit fly ATIC.
100μg
Verified Reactivity: Human
Reported Reactivity: Mouse, Rat, Frog, Drosophila
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ovalbumin-conjugated synthetic Peptide AHTNLRLFHH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2227799 (BioLegend Cat. No. 635602)
Structure: Belongs to the purH family, the IMP cyclohydrolase activity resides in the N-terminal region. Predicted molecular weight approximately 65 kD.
Distribution: Cytoplasm
Function: ATIC catalyzes the penultimate and final steps in the de novo purine biosynthesis pathway. ATIC acts as a bifunctional enzyme catalyze the formation of FAICAR and IMP.
Interaction: CDK2, Stomatin, JAK1, NM23B, IKKE, HNRNP, CIA30
Biology Area: Cell Biology
Antigen References: 1. Rayl EA, et al. 1996. J. Biol. Chem. 271:2225
Gene ID: 471
UniProt: View information about ATIC on UniProt.org
Clone: F38P7H9
Regulatory Status: RUO
Other Names: PURH, IMPCHASE/AICARFT, Bifunctional purine biosynthesis protein PURH
Isotype: Mouse IgG1, κ