Biolegend, 636802, Purified anti-p97/VCP Antibody, 100μg

p97/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. Clone 4G9 has been shown to be useful for western blotting, immunoprecipitation, and immunofluorescence staining of human p97/VCP protein.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human valosin containing protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, ICC - Reported by the developer, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 mL antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Shchedrina VA, et al. 2011. J Biol Chem. 186:42937. PubMed. Yi L, et al. 2012. Hum Mol Genet. 21:1794. PubMed. Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed.
Product Citations: Yi L, et al. 2012. Hum Mol Genet. 2.120833333. PubMed Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed Shchedrina V, et al. 2011. J Biol Chem. 286:42937. PubMed Efimova I, et al. 2021. Front Immunol. 12:643144. PubMed
RRID: AB_2257136 (BioLegend Cat. No. 636801) AB_2214641 (BioLegend Cat. No. 636802)
Structure: Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm diameter and displays 6-fold radial symmetry.
Interaction: VCIP135, SELS/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP
Bioactivity: VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Neuroscience, Neuroscience Cell Markers
Molecular Family: Endoplasmic Reticulum Markers
Antigen References: 1. Ye Y, et al. 2001. Nature 414:652.
Gene ID: 7415
UniProt: View information about p97/VCP on UniProt.org
Clone: 4G9
Regulatory Status: RUO
Other Names: Transitional endoplasmic reticulum ATPase, p97, TERA
Isotype: Mouse IgG1, κ