Biolegend, 637152, Purified anti-EGFR Phospho (Tyr1045) Antibody, 200microl

Epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the protein. Phosphorylation at tyrosine 1045 allows for binding directly to the adaptor protein GRB2. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.
200microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Synthetic peptide corresponding to the mature form of human EGFR phosphorylated at tyrosine 1045
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:400 - 1:4000. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone Poly23A05) is intended as a replacement for another antibody against the same target (clone Poly6563). Poly23A05 has a higher affinity for the target protein than Poly6563.
RRID: AB_3097617 (BioLegend Cat. No. 637151) AB_3097617 (BioLegend Cat. No. 637152)
Structure: EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.
Distribution: Plasma membrane
Function: EGFR signaling, cell signaling
Interaction: GRB2, SOS1, STAT1, STAT3, JAK2, CRK
Ligand/Receptor: Epidermal growth factor (EGF)
Cell Type: Epithelial cells
Biology Area: Cell Biology, Cell Proliferation and Viability, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Hunter T and Cooper JA. 1981. Cell. 24:741-52. Gill GN and Lazar CS. 1981. Nature. 293:305-7. Reynolds FH, et al. 1981. Nature. 292:259-62. Biscardi JS, et al. 1999. J Biol Chem. 274:8335-42. Zhou M, et al. 2013. Cancer Res. 23:7056-67-67.
Gene ID: 1956
UniProt: View information about EGFR on UniProt.org
Clone: Poly23A05
Regulatory Status: RUO
Other Names: Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA
Isotype: Rabbit Polyclonal IgG