Biolegend, 637551, Purified anti-Tubulin-β 2B (TUBB2B) Recombinant Antibody, 25μg

Tubulin is a major component of microtubules. It exists as a heterodimer consisting of an alpha and beta subunit. Class II beta tubulin is the major form of tubulin beta in neurons and is found in many other tissues including lung tissue and Schwann cells. TUBB2B is also indicated in various cancers and has been shown to have increased expression in hepatocellular carcinomas.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Recombinant peptide of Tubulin-B 2B
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For the use of this antibody (clone 7B9.rec) in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval with either Tris-EDTA (10 mM Tris, 1 mM EDTA, pH 9.0) or Citrate Buffer, 10X (Cat. No. 420902). For the use of this antibody (clone 7B9.rec) in immunocytochemistry (ICC), it is recommended to perform fixation/permeabilization with either 100% ice-cold methanol or fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 100% ice-cold methanol.
RRID: AB_3662318 (BioLegend Cat. No. 637551) AB_3662318 (BioLegend Cat. No. 637552)
Structure: TUBB2B is a 455 amino acid protein with a predicted molecular weight of 50 kD.
Distribution: Cytoplasm, cytoskeleton, and microtubules
Function: Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microtubules
Antigen References: Moody SA, et al. 1996. J Comp Neuro. 364: 219-30. Dozier JH, et al. 2003. Breast Cancer Res. 5:R157-69.
Gene ID: 347733
UniProt: View information about Tubulin-beta on UniProt.org
Clone: 7B9.Rec
Regulatory Status: RUO
Other Names: Tubulin beta-2B chain, class IIb beta-tubulin, class II beta-tubulin isotype, tubulin, beta polypeptide paralog
Isotype: Mouse IgG1, κ