Biolegend, 638051, Purified anti-PLCγ1 Antibody, 25μg

Phospholipase C gamma 1 (PLCγ1) is a signaling enzyme involved in intracellular signal transduction. In response to activation of receptor-type tyrosine kinases, phospholipase C (PLC) enzymes cleave the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2) into second messengers, namely inositol 1,4,5-triposphate (IP3) and diacylglycerol (DAG). IP3 causes the release of calcium from the endoplasmic reticulum to begin an intracellular signaling cascade, while DAG activates Protein Kinase C. There are four families of PLCs. The PLCγ family, consisting of PLCγ1 and PLCγ2 in humans, uniquely contains SRC domains SH2 and SH3. Receptor tyrosine kinases interact with these SRC domains to enzymatically activate PLCγ1 via phosphorylation. PLCγ1 is essential for cell proliferation, differentiation, and motility. Overexpression or activation of PLCγ1 has been implicated in breast and prostate cancers as well as cancer cell invasion.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the C-terminus of human PLCγ1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone W23013A) does not cross-react with mouse PLCγ1 in Western Blot. This antibody (clone W23013A) is not suitable for either immunocytochemistry (ICC) or intracellular flow cytometry (ICFC) due to dim signal. For immunohistochemistry (IHC), we recommend antigen retrieval with Sodium Citrate (Cat. No. 928502). Antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) is not recommended due to non-specific staining.
RRID: AB_3106246 (BioLegend Cat. No. 638051) AB_3106246 (BioLegend Cat. No. 638052)
Structure: PLCγ1 is a 1290 amino acid protein with a predicted molecular weight of 148.5 kD.
Distribution: Cytoplasmic
Function: Enzyme
Interaction: GRB2, and various Receptor tyrosine kinases (RTKs) such as PDGFRA, PDGFRB, EGFR, RET, FLT1, TRKA, BLNK, etc.
Cell Type: T cells
Biology Area: Cell Proliferation and Viability, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Ji QS, et al. 1997. Proc Natl Acad Sci. 94:2999-3003. Jang HJ, et al. 2018. Adv Biol Regul. 67:179-189. Yang YR, et al. 2016. Adv Biol Regul. 61:80-5.
Gene ID: 5335
UniProt: View information about PLCgamma1 on UniProt.org
Clone: W23013A
Regulatory Status: RUO
Other Names: PLCG1, 1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1, phospholipase C, gamma 1 (PLCG1), phospholipase C-II (PLC-II)
Isotype: Rat IgG2a, κ