Biolegend, 638354, Ultra-LEAF™ Purified anti-human BAFF (CD257) Antibody, 1mg

BAFF is a TNF-α cytokine member (a type II membrane protein) that acts in both a membrane-bound form and soluble cytokine form. The extracellular domain of BAFF shows high homology with APRIL. The soluble form is derived by cleavage in a furin consensus site. In human neutrophils treated with G-CSF, BAFF is processed intracellularly by a furin-type convertase. BAFF acts mainly as a soluble trimer and binds to three receptors with decreasing affinity in the following order: BAFFR > TACI > BCMA. Mice deficient in BAFF or its receptor BAFFR exhibit an obstruction in B cell development after the transitional T1 stage and lack marginal zone (MZ) and conventional B2 B cells. BAFFR-deficient mice and humans (patients with common variable immunodeficiency, or CVID) develop severe B cell lymphopenia due to the arrest of B cell development at the transitional B cell stage. Extreme BAFF production triggers severe autoimmune disorders in mice similar to Systemic Lupus Erythematosus (SLE) and Sjögren’s syndrome. BAFF has been associated with different human diseases such as pancreatic ductal adenocarcinoma (PDAC), autoimmune pancreatitis, SLE, rheumatoid arthritis, Sjögren's syndrome, lymphoid cancers, HIV infection, and B cell malignancies such as non-Hodgkin’s lymphoma (NHL).
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human BAFF
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the proliferation of primary mouse B cells induced by recombinant human BAFF (Cat. Nos. 559602, 559604, 559606, and 559608) at a spike concentration determined from a standard curve. ND50 range: 4 - 24 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3662320 (BioLegend Cat. No. 638353) AB_3662320 (BioLegend Cat. No. 638354)
Structure: Trimer
Distribution: Monocytes, macrophages, dendritic cells, neutrophils, and stromal cells of lymphoid organs
Function: BAFF induces B cell maturation, proliferation, survival, and immunoglobulin production. BAFF controls the size and composition of mature preimmune B cell pools. BAFF is induced by INF-γ in monocytes.
Interaction: Immature B cells, peripheral B-cells, naïve and memory cells
Ligand/Receptor: BAFFR, TACI (transmembrane activator and calcium-modulator and cyclophilin ligand interactor), and BCMA (B cell maturation antigen)
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction, Stem Cells
Molecular Family: CD Molecules, Cytokines/Chemokines, Growth Factors, Soluble Receptors
Antigen References: Schneider P, et al. 1999. J Exp Med. 189:1747-56. Nardelli B, et al. 2001. Blood. 97:198-204. Schiemann B, et al. 2001. Science. 5537:2111-4. Scapini P, et al. 2003. J Exp Med. 197:297-302. Yeramilli VA and Knight KL. 2010. J Immunol. 184:5527-36. Scholz JL and Cancro MP. 2012. Immunol. Lett. 143:2-8. Sindhava VJ, et al. 2013. Front Immunol. 4:37. Koizumi M, et al. 2013. PLoS One. 8:e71367.
Gene ID: 10673
UniProt: View information about BAFF on UniProt.org
Clone: W20066D
Regulatory Status: RUO
Other Names: B cell activating factor belonging to the TNF family (BAFF), B lymphocytes stimulator (BLyS), TNF- and APOL-related leucocyte expressed ligand 1(TALL1), TNF homolog that activates apoptosis, NKFB, JNK (THANK), TNF13B, CD257
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.