Biolegend, 639252, Purified anti-CD340 (ErbB2/HER-2) Phospho (Tyr1196) Antibody, 100μg

CD340, also commonly known as HER-2 or ErbB2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two CheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in lung adenocarcinoma, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. CD340 is a therapeutic target in several human cancers and it has been shown that overexpression in breast cancers confer Taxol resistance. CD340 autophosphorylation at Tyr1196 mediates Crk interaction that leads to Erk activation. In addition, CD340 phosphorylation has been related with polarity disruption, cell migration and metastasis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human CD340 phosphorylated at Tyr1196
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3662323 (BioLegend Cat. No. 639251) AB_3662323 (BioLegend Cat. No. 639252)
Structure: CD340 is a 1,255 amino acid protein with a predicted molecular weight of 185 kD. It is a glycosylated receptor-like transmembrane protein.
Distribution: Cellular membrane and also present in internalized vesicles
Function: Receptor, proto-oncogene
Interaction: Homodimers Heterodimers with EGFR, ErbB3, ErbB4 PIK3C2B, KPNB1, RANBP2, EEA1, CRM1, CLTC, MUC1, PTK6, RPA194, ACTB, PRKCABP, SRC, MYOC, CPNE3, SORL1, SH3BGRL
Ligand/Receptor: Epidermal growth factor family
Biology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability
Molecular Family: Adaptor Proteins, CD Molecules, Phospho-Proteins
Antigen References: Akiyama T, et al. 1986. Science. 232:1644-6. Bargmann CI, et al. 1986. Nature. 319:226-30. Pegram MD, et al. 1997. Oncogene. 15:537-47. Slamon DJ, et al. 1989. Science. 244:707-12. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID: 2064
UniProt: View information about CD340 on UniProt.org
Clone: A22023C
Regulatory Status: RUO
Other Names: Receptor tyrosine-protein kinase ErbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin
Isotype: Mouse IgG1, κ