Biolegend, 639354, Ultra-LEAF™ Purified anti-human IL-22RA2 (IL-22BP) Antibody, 1mg

IL-22BP is a soluble class 2 cytokine receptor family member (CRF2) without transmembrane and cytosolic domains.  IL-22BP binds to IL-22 and does not bind to other members of the IL-10 family. IL-22 signals through the mechanism that involves the sequential binding of IL-22 to interleukin-22 receptor 1 (IL-22R1), and of this dimer to interleukin-10 receptor 2 (IL-10R2) extracellular domain.  IL-22R1 expression is restricted to epithelial cells, hepatocytes, and acinar cells of the pancreas, whereas IL-10Rβ1 expression is ubiquitous. IL-22BP prevents the binding of IL-22 to the membrane bound IL-22R1. IL-22 has high affinity binding for IL-22BP, and it is 20- to 1000-fold higher compared to its binding to the membrane bound IL-22R1. IL-22BP inhibits IL-22 biological effects in vitro and in vivo and is constitutively expressed in secondary lymphoid organs, breast, and epithelial tissues such as gut, lung, and skin.IL-22BP plays a key role in controlling tumorigenesis and epithelial cell proliferation in the colon. High expression of IL-22BP has been detected in gastric adenoma tissues. In addition, non-affected skin of psoriasis patients shows lower expression of IL-22BP than skin of healthy controls; this decrease in IL-22BP exacerbates psoriatic skin inflammation.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human IL-22Rα2
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing human IL-22Rα2 (Cat. No. 766502) inhibition of IL-10 production induced by recombinant human IL-22 (Cat. No. 571302) at 0.2 µg/mL and 1 ng/mL on human Colo 205 cells. ND50 range: 0.1 - 0.7 μg/mL.
RRID: AB_3716898 (BioLegend Cat. No. 639353) AB_3716898 (BioLegend Cat. No. 639354)
Structure: Homodimer (soluble receptor)
Distribution: Activated B cell, monocytes, dendritic cells, and epithelial cells. It is induced by retinoic acid in immature monocyte-derived DC, and it is downregulated in mature DC.
Function: Antagonize the activity of IL-22, regulation of inflammatory responses. It is down regulated by IL-18 in intestinal tissue damage.
Ligand/Receptor: IL-22 (IL-10-related T cell-derived inducible factor, IL-TIF)
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors, Soluble Receptors
Antigen References: Dumoutier L, et al. 2001. J Immunol. 166:7090-5. Xu W, et al. 2001. Proc. Natl Acad Sci U S A. 98:9511-6. Logsdon NJ, et al. 2002. J Interferon Cytokine Res. 22:1099-112. Bleicher L, et al. 2008. FEBS Lett. 582:2985-92. Zheng Y, et al. 2008. Nat Med. 14:282.-9. Huber S, et al. 2012. Nature. 491:259-63. Martin JC, et al. 2014. Mucosal Immunol. 7:101-13. Yang SB, et al. 2016. Oncol Lett. 12:1477-1484. Martin JC, et al. 2017. J Immunol. 198:3671-3678.
Gene ID: 116379
UniProt: View information about IL-22RA2 on UniProt.org
Clone: W21014D
Regulatory Status: RUO
Other Names: Interleukin-22 Receptor Subunit Alpha-2, IL-22 receptor-alpha 2, IL-22R-alpha-2, IL-22RA2, Interleukin 22-Binding Protein, 22 Receptor Subunit Alpha-2, IL-22BP, Cytokine Receptor Family 2 Member 10, IL-22 Receptor Subunit Alpha-2
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.