Biolegend, 640252, Purified anti-CD73 Antibody, 100μg

CD73 (cluster of differentiation 73) is a membrane-bound glycoprotein and ectoenzyme. To activate adenosine signaling CD73 catalyzes the hydrolysis of AMP generated by CD39 to adenosine. This role makes CD73 crucial to the regulation of purinergic signaling and immune homeostasis and response. CD73 also serves a variety of physiological functions including lymphocyte binding, epithelial ion transport, ischemic preconditioning, tissue-barrier function, T cell activation, and tissue homeostasis. Overexpressed in several cancers and implicated in tumor progression, metastasis, and the impairment of anti-tumor immune response via adenosine signaling, CD73 is a target of interest in cancer therapy. It is also a potential immunotherapy target for the treatment of pain, inflammation, and hypoxia.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein corresponding to the nucleotidase domain of human CD73
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only This product is not recommended for use in immunohistochemistry in formalin fixed, paraffin-embedded tissues (IHC-P) or Western blot (WB).
RRID: AB_3717149 (BioLegend Cat. No. 640251) AB_3717149 (BioLegend Cat. No. 640252)
Structure: CD73 is a 574 amino acid protein with a predicted molecular weight of 63 kD.
Distribution: Cell membrane
Function: Hydrolase, metabolic protein, CD marker, enzyme
Interaction: AP-2, SMAD, SP-1, CD39, GSDMD
Ligand/Receptor: Metal-binding, nucleotide-binding, zinc
Cell Type: Lymphocytes, T cells
Biology Area: Cell Biology
Molecular Family: CD Molecules, Enzymes and Regulators, Innate Immune Signaling
Antigen References: Knapp K, et al. 2012. Structure. 20:2161. Minor M, et al. 2019. Am. J. Physiol. Cell Physiol. 317:C1079. Yu M, et al. 2020. Nat. Commun. 11:515.
Gene ID: 4907
UniProt: View information about CD73 on UniProt.org
Clone: W23165D
Regulatory Status: RUO
Other Names: clulster of differentiation 73 (CD73), 5'-Nucleotidase Ecto (NT5E), CALJA, ENT, NT5, EN, E5NT
Isotype: Rat IgG2a, κ