Biolegend, 642351, Purified anti-BLK Antibody, 25μg

BLK is a non-receptor tyrosine kinase primarily expressed in B lymphocytes and other cells of the B-cell lineage. BLK belongs to the Src family of kinases and plays a critical role in B cell receptor signaling, which is essential for the activation, differentiation, and proliferation of B cells. Phosphorylation of target proteins by BLK helps transmit signals that guide B cell development and activation. Dysregulation of BLK has been associated with various immune disorders, including autoimmune diseases and certain types of B cell malignancies. Outside of B cells, BLK is also expressed in a variety of immune and non-immune cells including plasmacytoid dendritic cells, pancreatic β cells, and bone marrow progenitor cells.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant full-length human BLK
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product (clone W21005C) may cross-react with other members of the Src kinase family. This product (clone W21005C) is not recommended for use in immunocytochemistry (ICC) or immunohistochemistry (IHC). For use in intracellular flow cytometry (ICFC), permeabilization with True-PhosTM Perm Buffer (Cat. No. 425401) is recommended. Fixation/permeabilization with Cyto-FastTM Fix/Perm Buffer Set (Cat. No. 426803) is not recommended. This antibody does not cross react with mouse BLK in Western Blot (WB).
RRID: AB_3675177 (BioLegend Cat. No. 642351) AB_3675177 (BioLegend Cat. No. 642352)
Structure: BLK is a 505 amino acid protein with a predicted molecular weight of 57.7 kD.
Distribution: Cytoplasmic, plasma membrane
Function: Kinase
Interaction: Phosphorylates a variety of targets including BLNK, IRF3, SYK, and ITAM-containing proteins such as CD79a and CD79b
Cell Type: B cells, Leukemia
Biology Area: Cell Biology, Immuno-Oncology, Immunology
Antigen References: Dymecki SM, et al. 1990. Science. 247:332-6. Zhang H, et al. 2012. Nat Genet. 44:861-71. Malek SN, et al. 1998. Proc Natl Acad Sci USA. 95:7351-6. Akerblad P, et al. 1999. J Immunol. 163:5453-61.
Gene ID: 640
UniProt: View information about BLK on UniProt.org
Clone: W21005C
Regulatory Status: RUO
Other Names: Tyrosine-protein kinase Blk, B lymphocyte kinase, p55-BLK
Isotype: Rat IgG2b, κ