Biolegend, 643808, Direct-Blot™ HRP anti-β-actin Antibody, 25microl

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
25microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.
Application References(PubMed link indicates BioLegend citation): Lawson BR, et al. 2007. J. Immunol. 178:5366. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053. Yanagiya T,et al.2007.Obesity.15:572. KishidaT,et al. 2007.J. Immunol. 179:8554. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. Toltlt LJ,et al. 2008.J. Immunol. 181:2165. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. Ikeda D, et al. 2008. Endocrinology. 149:6037. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
Product Citations: Hochrein SM, et al. 2022. Cell Metab. 34:516. PubMed Eguiarte-Solomon F, et al. 2021. Mol Carcinog. 60:342. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Kyle Cottrell, Sergej Djuranovic 2016. PLoS One. 11:e0167877. PubMed Vassilieva EV, et al. 2020. Front Immunol. 2.504166667. PubMed Doi T, et al. 2022. Heliyon. 8:e08890. PubMed Zacks DN, et al. 2022. J Clin Med. 11:. PubMed Wang J, et al. 2021. Biomolecules. 11:. PubMed
RRID: AB_2734515 (BioLegend Cat. No. 643808) AB_2566701 (BioLegend Cat. No. 643807)
Structure: Actin, cytoplasmic 1
Distribution: Ubiquitously expressed in the cytoplasm of all eukaryotic cells
Function: Actins are highly conserved proteins that are involved in cell motility
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microfilaments
Antigen References: 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
Gene ID: 11461
UniProt: View information about beta-actin on UniProt.org
Clone: 2F1-1
Regulatory Status: RUO
Other Names: Actin, cytoplasmic 1
Isotype: Mouse IgG2b, κ