Biolegend, 644003, PE anti-Cyclin A Antibody, 25tests

Cyclins regulate the activity of the Cyclin-dependent protein kinases. Cyclin A is involved in the regulation of the cell cycle and seems to be essiential for S phase and M phase. Studies have shown that overexpression of cyclin A can accelerate the G1 to S phase. Cyclin A is a marker for actively proliferating cells and for cells in S phase.
25tests
Verified Reactivity: Human, Mouse
Reported Reactivity: Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Cyclin A fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Antibody may perform better for ICFC if cells sit in 70% ethanol or True Phos™ perm Buffer for 24 hours – 72 hours at -20° C
RRID: AB_2715836 (BioLegend Cat. No. 644003) AB_2715837 (BioLegend Cat. No. 644004)
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: 1. Pagano M et al. 1992. EMBO J 11:961 2. Geley S et al. 2001 J Cell Biol. 153:137 3. Resinitzky D et al. 1995. Mol Cell Biol 15:4347 4. Furuno N et al. 1999. J Cell Biol. 147:295
Gene ID: 890
UniProt: View information about Cyclin A on UniProt.org
Clone: E23.1
Regulatory Status: RUO
Other Names: CCNA2, cyclin A2, CCN1, CCNA
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.