Biolegend, 644105, PE anti-CD247 (TCRζ, CD3ζ) Antibody, 25μg

The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TCR-zeta peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.2 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Zhang Z, et al. 2007. Blood 109:4328. (Block) Gorman CL, et al. 2008. J. Immunol. 180:1060. Jenson WA, et al. 1997. Eur J. Immunol. 27:707. Mao H, et al. 2010. J. Virol. 84:4148. PubMed Hwang I, et al. 2012. Int Immunol. 24:793. PubMed.
Product Citations: Lah S, et al. 2023. J Immunother Cancer. 11:. PubMed Woan KV, et al. 2021. Cell Stem Cell. 28:2062. PubMed Hintz HM, et al. 2021. Cancer Immunol Res. 9:1270. PubMed Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
RRID: AB_2565720 (BioLegend Cat. No. 644105) AB_2565721 (BioLegend Cat. No. 644106)
Disclaimer: This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Structure: Structure: 163 amino acid long protein (predicted MW is 18kDa), with a short extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution: T cells and NKT cells. Reported also positive on CD16+ human NK cells.
Function: Mediating signal transduction after TCR receptor engagement.
Interaction: ZAP70, TCR alpha/beta
Cell Type: NKT cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules, TCRs
Antigen References: 1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709
Gene ID: 919
UniProt: View information about CD247 on UniProt.org
Clone: 6B10.2
Regulatory Status: RUO
Other Names: CD247, CD3zeta
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.