Biolegend, 644803, Alexa Fluor® 647 anti-T-bet Antibody, 25μg

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Application References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations: Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed Zutshi S, et al. 2021. Immunology. 163:460. PubMed Mukherjee A, et al. 2021. Immunology. 164:318. PubMed Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed Wang D, et al. 2018. Immunity. 48:659. PubMed Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed Snyder LM, et al. 2022. Immunohorizons. 6:660. PubMed Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed Soderquest K, et al. 2017. PLoS Genet. 13(2):e1006587. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Vogel AB, et al. 2021. Nature. 592:283. PubMed Camell CD, et al. 2020. Cell Metabolism. 30(6):1024-1039.e6.. PubMed Yan J, et al. 2020. Cell Rep. 107820:31. PubMed Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed Doss PMIA, et al. 2021. Cell Reports. 34(10):108833. PubMed Wieten R, et al. 2016. PLoS One. 11: 0149871. PubMed Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed Chmielewski M and Abken H 2017. Cell Rep.. 10.1016/j.celrep.2017.11.063. PubMed Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
RRID: AB_1595573 (BioLegend Cat. No. 644803) AB_1595466 (BioLegend Cat. No. 644804)
Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009
UniProt: View information about T-bet on UniProt.org
Clone: 4B10
Regulatory Status: RUO
Other Names: T-box expressed in T cells, T box 21, TBLYM
Isotype: Mouse IgG1, κ