Biolegend, 644809, PE anti-T-bet Antibody, 25μg

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a staining volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Application References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations: Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed Cheng C, et al. 2022. Cell Mol Gastroenterol Hepatol. 15:261. PubMed Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed Dölz M, et al. 2022. iScience. 25:105372. PubMed Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Denis M, et al. 2023. Front Immunol. 13:1011943. PubMed Zhou J, et al. 2019. Immunity. 50:403. PubMed Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed Tauriainen J, et al. 2017. Sci Rep. 7:40354. PubMed Chen D, et al. 2018. Nat Commun. 9:873. PubMed Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Werbner M, et al. 2019. mSystems. 4:e00292-18. PubMed Mao A,et al. 2017. Nat Commun. . 10.1038/s41467-017-00882-0. PubMed Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed Dionisio-Santos DA, et al. 2021. Front Neurosci. 15:758677. PubMed Śledzińska A, et al. 2020. Immunity. 52(1):151-166.e6.. PubMed Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed Dhar P, et al. 2021. Commun Biol. 4:905. PubMed Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed Webb LM, et al. 2020. J Clin Invest. 130:1683. PubMed Pieper R, et al. 2020. Gut Microbes. 12:1782163. PubMed
RRID: AB_2028583 (BioLegend Cat. No. 644809) AB_2200542 (BioLegend Cat. No. 644810)
Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009
UniProt: View information about T-bet on UniProt.org
Clone: 4B10
Regulatory Status: RUO
Other Names: T-box expressed in T cells, T box 21, TBLYM
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.