Biolegend, 644813, APC anti-T-bet Antibody, 25μg

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Application References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations: Chang D, et al. 2023. Front Immunol. 14:1105145. PubMed Wildner NH, et al. 2021. J Leukoc Biol. 109:77. PubMed Gringhuis SI, et al. 2022. Nat Immunol. 23:1735. PubMed Janeczek K, et al. 2023. Front Immunol. 14:1073788. PubMed Fiege JK, et al. 2021. Cell Host Microbe. 29:1815. PubMed Zander R, et al. 2022. Immunity. 55:475. PubMed Ye L, et al. 2022. Cell Metab. 34:595. PubMed Ren Z, et al. 2021. EMBO Molecular Medicine. :e14059. PubMed Cuadrado E, et al. 2018. Immunity. 48:1046. PubMed Romain Bouziat et al. 2018. Cell host & microbe. 24(5):677-688 . PubMed Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed Kaur K, et al. 2021. Cell Rep. 37:110178. PubMed Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed Wiede F, et al. 2021. Cancer Discov. Online ahead of print. PubMed Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed Holly Bachus et al. 2018. Immunity. 50(1):225-240 . PubMed Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed Snell LM, et al. 2018. Immunity. 49:678. PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed Woodruff M, et al. 2020. medRxiv. . PubMed Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed Wagner A, et al. 2021. Cell. . PubMed Webb ER, et al. 2022. iScience. 25:104995. PubMed LM S, et al. 2016. Cell Rep. 16(12): 3286-96. PubMed Lu Y, et al. 2018. Br J Pharmacol. 175:1085. PubMed Giampazolias E, et al. 2021. Cell. . PubMed Shemesh A, et al. 2022. J Exp Med. 219:. PubMed Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed Cao Y, et al. 2019. Nat Commun. 10:1280. PubMed Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed Kumar R, et al. 2020. Cell Reports. 30(8):2512-2525. PubMed
RRID: AB_10896913 (BioLegend Cat. No. 644813) AB_10901173 (BioLegend Cat. No. 644814)
Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009
UniProt: View information about T-bet on UniProt.org
Clone: 4B10
Regulatory Status: RUO
Other Names: T-box expressed in T cells, T box 21, TBLYM
Isotype: Mouse IgG1, κ