Biolegend, 644828, PE/Dazzle™ 594 anti-T-bet Antibody, 100μg

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a staining volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application. * PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Application References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations: Haugstøyl ME, et al. 2023. Front Immunol. 14:1130370. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Fitzgerald AA, et al. 2021. J Immunother Cancer. 9:. PubMed Runge EM, et al. 2020. J Neuroinflammation. 17:121. PubMed Stokes J, et al. 2020. Oncoimmunology. 9:1758011. PubMed Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed Harper JL, et al. 2020. Nat Med. 519:26. PubMed Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed Bajaña S, et al. 2022. iScience. 25:103732. PubMed
RRID: AB_2565676 (BioLegend Cat. No. 644827) AB_2565677 (BioLegend Cat. No. 644828)
Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009
UniProt: View information about T-bet on UniProt.org
Clone: 4B10
Regulatory Status: RUO
Other Names: T-box expressed in T cells, T box 21, TBLYM
Isotype: Mouse IgG1, κ