Biolegend, 644832, Brilliant Violet 421™ anti-T-bet Antibody, 50μg

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
50μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 0.4 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Application References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations: Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed Barber-Axthelm IM, et al. 2023. iScience. 26:106269. PubMed Pieren DK, et al. 2023. Nat Commun. 14:1887. PubMed Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed Glaubitz J, et al. 2023. Gut. :. PubMed Dean JW 2023. Cell Reports. 42(1):111963. PubMed Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed Otano I, et al. 2021. Nat Commun. 12:7296. PubMed Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed Aalderen M, et al. 2015. J Virol. 89:5110. PubMed Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed Wang HQ, et al. 2021. Cancer Research. 81(11):3079-3091. PubMed Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed Marquardt N, et al. 2019. Nat Commun. 10:3841. PubMed Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed Singh PP, et al. 2021. Ocul Surf. 21:271. PubMed Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Lorentsen KJ, et al. 2018. Nat Commun. 9:1679. PubMed Schaik B, et al. 2015. J Virol. 89:568. PubMed Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed Ganguly S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:891. PubMed Hombrink P, et al. 2016. Nat Immunol. 17:1467-1478. PubMed Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed Phalke SP, et al. 2019. PLoS One. 14:e0218827. PubMed Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed Cheng J, et al. 2022. iScience. 25:103588. PubMed Collins N, et al. 2020. Cell. 178(5):1088-1101.e15.. PubMed Berrien–Elliott MM, et al. 2020. Immunity. 51(3):479-490. PubMed Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed Aalderen M, et al. 2013. J Virol. 87:10263. PubMed Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed Goc J, et al. 2021. Cell. 184:5015. PubMed Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed Shao TY et al. 2019. Cell host & microbe. 25(3):404-417 . PubMed Song W, et al. 2022. Immunity. 55:290. PubMed
RRID: AB_10896427 (BioLegend Cat. No. 644815) AB_2686976 (BioLegend Cat. No. 644832) AB_10959653 (BioLegend Cat. No. 644816)
Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009
UniProt: View information about T-bet on UniProt.org
Clone: 4B10
Regulatory Status: RUO
Other Names: T-box expressed in T cells, T box 21, TBLYM
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.