Biolegend, 645803, FITC anti-p53 Antibody, 25tests

p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: p53 N-terminal linear epitope aa 20-25
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Vojtesek B, et al. 1992. J. Immunol. Methods 151:237. Stephen CW, et al. 1995. J. Mol. Biol. 248:58.
Product Citations: Dong Y, et al. 2020. J Leukoc Biol. 108:1711. PubMed Barili V, et al. 2020. Nat Commun. 0.877777778. PubMed Ladds MJGW, et al. 2018. Nat Commun. 9:1107. PubMed Kannan S, et al. 2019. Mol Cancer Ther. 1.871527778. PubMed
RRID: AB_2561798 (BioLegend Cat. No. 645803) AB_2561799 (BioLegend Cat. No. 645804)
Structure: Tetramer; 53 kD
Distribution: Nuclear when activated
Function: Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
Interaction: mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
Cell Type: Mesenchymal Stem Cells
Biology Area: Cell Biology, DNA Repair/Replication, Stem Cells
Gene ID: 7157
UniProt: View information about p53 on UniProt.org
Clone: DO-7
Regulatory Status: RUO
Other Names: Tumor protein p53 (TP53)
Isotype: Mouse IgG2b, κ