Biolegend, 646001, Purified anti-Syk Antibody, 25μg

Syk is a cytoplasmic 72 kD non-receptor tyrosine kinase that is widely expressed in hematopoietic cells. The Syk kinase associates with a number of proteins including c-Src, Lck, Lyn, Fyn, Vav1, STAT3, SHP1, Grb2, and LAT to couple immunoreceptors to downstream signaling events mediating cellular proliferation, differentiation, and phagocytosis. Syk is modified by phosphorylation on multiple tyrosine sites.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Syk peptide aa306-333
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Additional Product Notes: In the first WB figure above, the cell lines selected for antibody testing were chosen to demonstrate specificity and species cross-reactivity, A549 was chosen as a human control cell line that does not express Syk protein; RPMI 8225 and Daudi are human cell lines that express Syk protein; and A20 cells is a mouse cell line that expresses Syk protein. Predicted SYK expression was obtained from Human Protein Atlas.
Application References(PubMed link indicates BioLegend citation): Chu D, et al. 1999. J. Immunol. 163:2610. Ackermann JA, et al. 2015. J Immunol. 194:4650. PubMed
Product Citations: Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed Ackermann J, et al. 2015. J Immunol. 194:4650. PubMed Németh T, et al. 2016. Nat Commun. 7: 11004. PubMed
RRID: AB_1659262 (BioLegend Cat. No. 646001) AB_1659261 (BioLegend Cat. No. 646002)
Structure: Non-receptor associated tyrosine kinase, contains 2 SH2 domains, 72 kD
Distribution: Cytoplasmic protein, widely expressed in hematopoietic cells
Function: Couples immunoreceptor to downstream signaling events that mediate cellular responses including proliferation, differentiation, and phagocytosis
Biology Area: Cell Biology, Signal Transduction
Antigen References: 1. Taniguchi T, et al. 1991. J. Biol. Chem. 266:15790. 2. Toyabe SL, et al. 2001.Immunology 103:164.
Gene ID: 6850
UniProt: View information about Syk on UniProt.org
Clone: 5F5
Regulatory Status: RUO
Other Names: Spleen tyrosine kinase, protein tyrosine kinase SYK
Isotype: Mouse IgG1, κ