Biolegend, 646252, Purified anti-IRAK3 (IRAK-M) Antibody, 100μg

The interleukin-1 receptor-associated kinase (IRAK) family of proteins are made up of serine/threonine-specific kinases which play a critical role in the response to pathogens through the induction of acute inflammation and subsequent adaptive immune responses. The mammalian IRAK molecular family consists of four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). Two are active kinases, IRAK1 and IRAK4, and two are inactive kinases, IRAK2 and IRAK3. However, all are involved in the regulation of the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1R1, IL-1RAcP, MyD88, and IRAKs. Unlike its family members IRAK1 and IRAK4, which promote inflammatory responses, IRAK3 (IRAK-M) functions as a negative regulator, helping to maintain immune homeostasis and prevent excessive inflammation. It is predominantly expressed in monocytes, macrophages, and dendritic cells, where it plays an essential role in dampening TLR-mediated immune activation. IRAK3 prevents the dissociation of IRAK1 and IRAK4 from MyD88, thereby disrupting downstream activation of TRAF6. It also inhibits the phosphorylation and degradation of IRAK1, which is a crucial step in propagating TLR signaling. Moreover, IRAK3 suppresses NF-κB activation, reducing the transcription of inflammatory cytokines. This regulatory role is essential for preventing excessive inflammation and avoiding conditions such as sepsis and chronic inflammatory diseases. Loss of IRAK3 function has been associated with hyperinflammatory states, leading to conditions such as sepsis, acute lung injury, and inflammatory bowel disease.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human IRAK3 (IRAK-M)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verifed
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in intracellular flow cytometry (ICFC), it is recommended to fix with Fixation Buffer (Cat. No. 420801) and permeabilize with either of the following: -Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) -True-Phos™ Perm Buffer (Cat. No. 425401) -True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401)
RRID: AB_3699073 (BioLegend Cat. No. 646251) AB_3699073 (BioLegend Cat. No. 646252)
Structure: IRAK3 is a 596 amino acid length protein with a predicted molecular weight of 67.8 kD and has a 60.6 kD isoform.
Distribution: Cytoplasm
Cell Type: Dendritic cells, Eosinophils, Macrophages, Monocytes
Biology Area: Immuno-Oncology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Kobayashi K, et al. 2002. Cell. 110:191-202. Miyata M, et al. 2015. Nat Commun. 2015. 14:6:6062. Zhang C-J, et al. 2018. J Clin. Invest. 2018. 128:5399-5412. Liu W, et al. 2022. Nat Commun. 2022. 13:2748.
Gene ID: 11213
UniProt: View information about IRAK3 on UniProt.org
Clone: W23096A
Regulatory Status: RUO
Other Names: IRAK-3, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, IRAKM, Inactive IL-1 receptor-associated kinase 3, ASRT5
Isotype: Rat IgG2b, κ