Biolegend, 647504, PE anti-XBP-1s Antibody, 100tests

XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its ability to bind X-box, a conserved transcriptional element in the promoter of human HLA DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 upregulates as part of the ER stress response, also known as the unfolded protein response. Unspliced XBP-1 is 261 amino acids and migrates on the SDS-PAGE around 33 kD; spliced XBP-1, recognized by clone 143F, is 371 amino acids and migrates around 55 kD.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: XBP-1s recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunofluorescence14 and immunohistochemical staining of PFA-fixed paraffin-embedded sections1,14.
Application References(PubMed link indicates BioLegend citation): Tsang KY, et al. 2007. PLOS Biol. 5:568. (IHC) PubMed Farhan H, et al. 2008. EMBO J. 27:2043 (WB) PubMed Farhan H, et al. 2010. J. Cell Biol. 189:997. PubMed Feng-Jin G, et al. 2010. Cell Signal. [Epub ahead of print] Wang L, et al. 2011. Hum Mol Genet. PubMed Li J, et al. 2011. J. Biol Chem. 286:4912. PubMed Wang L, et al. 2011. Hum Mol Genet. 20:1008. PubMed Liu Y, et al. 2011. J Biol Chem. 286:13161. PubMed Shirley CM, et al. 2011 Blood. 117:6297. PubMed Vidal RL., et al. 2012. Hum Mol Genet. PubMed Byrd AE, et al. 2012. J Cell Biol. 196:689. PubMed Dickie LJ, et al. 2012. Ann rheum Dis. 71:2035. PubMed Bonetti P, et al. 2013. Blood. 122:2233. PubMed Maestre L, et al. 2009. Haematologica. 94:419. (IF, IHC) Rodriguez M, et al. 2014. J Biol Chem. 289:22942. PubMed
Product Citations: Katsuyama E, et al. 2024. Nat Commun. 8304:15. PubMed Grayson KA, et al. 2021. Mol Cancer Ther. 20:833. PubMed
RRID: AB_2563447 (BioLegend Cat. No. 647503) AB_2563448 (BioLegend Cat. No. 647504)
Distribution: Ubiquitously expressed.
Function: Upregulated by ER stress, IL-6, and IL-4. Downregulation correlates with tumor progression in prostate cancer. Downregulated by PAX5 transcription factor.
Biology Area: Cell Biology
Antigen References: 1. Liou HC, et al. 1990. Science 247:1581. 2. Ponath PD, et al. 1993. J. Biol. Chem.268:17074. 3. Takahashi S, et al. 2002. Prostate 50:154.
Gene ID: 7494
UniProt: View information about XBP-1s on UniProt.org
Clone: 143F
Regulatory Status: RUO
Other Names: X-box binding protein 1
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.