Biolegend, 647901, Purified anti-Cyclin B1 Antibody, 25μg

Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48 kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. The V152 monoclonal antibody recognizes human and mouse Cyclin B1 and has been shown to be useful for Western blotting and immunohistochemistry (paraffin embedded tissue sections) of human and mouse cyclin B1.
25μg
Verified Reactivity: Human
Reported Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Hamster Cyclin B1
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 2.5 - 5.0 µg antibody per 5 mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include: immunohistochemistry2 of formalin-fixed paraffin-embedded sections and immunofluorescence1 of methanol fixed cells grown on glass coverslips.
Application References(PubMed link indicates BioLegend citation): Geley S, et al. 2001. J. Cell Biol. 153:137-148 PubMed. Brandeis M, et al. 1998. P. Natl. Acad. Sci. USA 95:4344-4349. PubMed Matsumura F, et al. 2011. J. Biol Chem. PubMed
Product Citations: Brazzo JA, et al. 2021. J Cell Sci. 134:. PubMed Matsumura F, et al. 2011. J Biol Chem. 286:10825. PubMed Neumann-Staubitz P, et al. 2022. Front Chem. 10:905794. PubMed Iii JAB, et al. 2021. J Cell Sci. . PubMed
RRID: AB_2072274 (BioLegend Cat. No. 647901) AB_2244210 (BioLegend Cat. No. 647902)
Structure: Belongs to the cyclin family. Cyclin AB subfamily.
Distribution: Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.
Interaction: CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4
Bioactivity: Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: 1. Morgan DO, et al.1995. Nature 374:131
Gene ID: 891
UniProt: View information about Cyclin B1 on UniProt.org
Clone: V152
Regulatory Status: RUO
Other Names: CCNB1
Isotype: Mouse IgG1