Biolegend, 648002, Purified anti-Hsp70 Antibody, 100μg

Hsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits immunomodulatory effects on innate and acquired immunity.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Hsp70 protein from HeLa cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB, ICFC - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Fong J, et al. 2015. EMBO J. 34: 2775 - 2788. PubMed Zhang K, et al. 2021. Neuron. 109(12):1949-1962.e6. PubMed Våtsveen TK, et al. 2018. J Hematol Oncol. 11:23. PubMed Harris IS et al. 2019. Cell metabolism. 29(5):1166-1181 . PubMed Hashimoto M, et al. 2022. Front Neurosci. 16:948517. PubMed
RRID: AB_2028531 (BioLegend Cat. No. 648001) AB_2264228 (BioLegend Cat. No. 648002)
Structure: Hsp proteins are the most conserved proteins present in both prokaryotes and eukaryotes. Human HSp70 contains multiple isoforms. Hsp70s consisit of three structural domains, a 44Kd amino-terminal ATPase domain (NBD), followed by an 18 Kd substrate
Distribution: Ubiquitous, cytoplasmic and nuclear
Function: Molecular chaperrones assisting in the folding of proteins and preventing protein aggregation
Biology Area: Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
Antigen References: 1. Sharma D, et al. 2009. Protein Peptide Lett. 16:571. 2. Johnson JD, et al. 2006. J. Leuk. Biol. 79:425. 3. Bukau B, et al. 1998. Cell 92:351.
Gene ID: 3308
UniProt: View information about Hsp70 on UniProt.org
Clone: W27
Regulatory Status: RUO
Other Names: Heat shock protein 70
Isotype: Mouse IgG2a, λ