Biolegend, 648005, Direct-Blot™ HRP anti-Hsp70 Antibody, 100microl

Hsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits immunomodulatory effects on innate and acquired immunity.
100microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Hsp70 protein from HeLa cells.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
RRID: AB_2566707 (BioLegend Cat. No. 648005)
Structure: Hsp proteins are the most conserved proteins present in both prokaryotes and eukaryotes. Human HSp70 contains multiple isoforms. Hsp70s consisit of three structural domains, a 44Kd amino-terminal ATPase domain (NBD), followed by an 18 Kd substrate
Distribution: Ubiquitous, cytoplasmic and nuclear
Function: Molecular chaperrones assisting in the folding of proteins and preventing protein aggregation
Biology Area: Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
Antigen References: 1. Sharma D, et al. 2009. Protein Peptide Lett. 16:571. 2. Johnson JD, et al. 2006. J. Leuk. Biol. 79:425. 3. Bukau B, et al. 1998. Cell 92:351.
Gene ID: 3308
UniProt: View information about Hsp70 on UniProt.org
Clone: W27
Regulatory Status: RUO
Other Names: Heat shock protein 70
Isotype: Mouse IgG2a, λ