Biolegend, 648152, Purified anti-LDHA Antibody, 100μg

L-lactate dehydrogenase A chain, also known as LDHA, is an enzyme that plays a key role in cellular energy metabolism, particularly under anaerobic conditions. LDHA catalyzes the conversion of pyruvate to lactate, which coupled with the oxidation of NADH to NAD+ is essential for maintaining glycolysis when oxygen is limited. LDHA is widely expressed, with the highest levels in tissues with high energy demands such as muscle. In cancer cells, LDHA supports the Warburg effect by promoting lactate production, thus LDHA is a potential target for cancer therapy due to its role in tumor progression and metabolic reprogramming. This product was carefully developed for performance and specificity using the following product from Horizon Discovery, a Revvity company: Human HAP1 Knockout Cell Line – LDHA (Horizon Cat. No. HZGHC004917c008)
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human LDHA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: - Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol - 100% ice-cold methanol only Note: Fixation/ permeabilization with Fixation buffer (Cat. No. 420801) followed by 0.5 % Triton X-100 is not recommended due to dim signal. For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) or True-Phos™ Perm Buffer (Cat. No. 425401). Signal is brighter using True-Phos™ Perm Buffer. Note: Fixation/permeabilization with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended due to dim signal.
RRID: AB_3699075 (BioLegend Cat. No. 648151) AB_3699075 (BioLegend Cat. No. 648152)
Structure: LDHA is a 332 amino acid protein with a predicted molecular weight of 36.7 kD.
Distribution: Cytoplasm
Interaction: HSP90: Helps stabilize and fold LDHA, particularly under stress conditions GAPDH: LDHA forms complexes with other glycolytic enzymes to facilitate metabolism AKT: AKT phosphorylates LDHA, influencing its activity
Biology Area: Cancer Biomarkers, Cell Biology
Molecular Family: Enzymes and Regulators
Antigen References: Feng Y, et al. 2018. Cancer Med. 7:6124-6136. Sharma, et al. 2022. Semin Cancer Biol. 87:184-195. Urbanska K and Orzechowski A. 2019. Int J Mol Sci 20:2085. Kraus AP and Neely CJ. 1964. Science. 145:595-7.
Gene ID: 3939
UniProt: View information about LDHA on UniProt.org
Clone: A22115F
Regulatory Status: RUO
Other Names: L-lactate dehydrogenase A chain (LDHA), cell proliferation inducing gene 19, LDH muscle subunit (LDH-M), Renal carcinoma antigen NY-REN-59, PIG19
Isotype: Mouse IgG2b, κ