Biolegend, 648552, Purified anti-TRIM29 Antibody, 100μg

TRIM29 (Tripartite Motif Containing 29), also known as ATDC (Ataxia-Telangiectasia Group D-Complementing), is a member of the TRIM protein family, which is characterized by a tripartite motif consisting of a RING finger domain, one or two B-box motifs, and a coiled-coil domain. Unlike most TRIM proteins, TRIM29 lacks the typical RING finger domain, which often confers E3 ubiquitin ligase activity. TRIM29 is primarily expressed in epithelial tissues such as the skin, gastrointestinal tract, and respiratory system. In normal physiological conditions, TRIM29 plays a crucial role in maintaining genomic stability and is involved in DNA repair pathways interacting with molecules such as p53-binding protein 1 (53BP1) and TIP60. Through these interactions, TRIM29 helps regulate DNA double-strand break (DSB) repair via the non-homologous end joining (NHEJ) and homologous recombination (HR) pathways. TRIM29 also functions in controlling epithelial cell differentiation and immune responses. TRIM29 can suppress type I interferon responses by regulating the STING pathway, which is essential for antiviral defense. In breast cancer and head and neck squamous cell carcinoma (HNSCC), TRIM29 often acts as an oncogene. Overexpression of TRIM29 in these cancers promotes tumor growth, invasion, and metastasis. Conversely, in gastric and colorectal cancers, TRIM29 act as a tumor suppressor. The expression is often reduced or silenced via promoter hypermethylation. The loss of TRIM29 function may lead to increased genomic instability and tumor progression.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human TRIM29
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).
RRID: AB_3717123 (BioLegend Cat. No. 648551) AB_3717123 (BioLegend Cat. No. 648552)
Structure: Contains 588 amino acids with a predicted molecular weight of 66 kD.
Distribution: Cytoplasm
Cell Type: Epithelial cells, Macrophages
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Ubiquitin/Protein Degradation
Antigen References: Masuda Y, et al. 2015. Nat. Commun. 6:7299. Xing J. et al. 2016. Nat. Immunol.17:1373-1380. Li Q, et al. 2018. Cell Discov. 4:13.
Gene ID: 23650
UniProt: View information about TRIM29 on UniProt.org
Clone: W21025C
Regulatory Status: RUO
Other Names: Tripartite motif-containing protein 29, Ataxia telangiectasia group D-associated protein, ATDC
Isotype: Rat IgG2a, κ