Biolegend, 648652, Purified anti-DAP12 (TYROBP) Antibody, 100μg

DAP12, also known as TYROBP, is a transmembrane adapter protein expressed in various immune cells, including natural killer cells, monocytes, macrophages, dendritic cells, osteoclasts, and microglia. It plays a role in immune cell functions such as survival, proliferation, phagocytosis, chemotaxis, and cytokine production. DAP12 forms complexes with several immunoreceptors, including TREM2, to trigger intracellular signaling pathways. Regulation of DAP12 involves its interaction with proteins like RER1, which retains unassembled DAP12 in early secretory compartments, preventing its transport to the plasma membrane. Mutations in DAP12 and its associated receptors have been linked to neurodegenerative diseases, such as Nasu-Hakola disease and Alzheimer's disease. Additionally, DAP12 signaling can modulate immune responses, as seen in its paradoxical role in down-regulating cytokine production by plasmacytoid dendritic cells during viral infections.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human DAP12
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using Cyto-Fast™ Fix-Perm Buffer Set (Cat. No. 426803). We do not recommend True-Phos™ Perm Buffer (Cat. No. 425401).
RRID: AB_3717125 (BioLegend Cat. No. 648651) AB_3717125 (BioLegend Cat. No. 648652)
Structure: DAP12 is composed of 113 amino acids with a molecular weight approximately 12.2 kDa.
Distribution: Mainly located in the plasma membrane. Also, it can be found in the endoplasmic reticulum and in the ER-golgi intermediate compartment.
Function: • Immune Cell Activation: DAP12 is crucial for activating various immune cells, including natural killer cells, macrophages, and dendritic cells. • Signal Transduction: It forms complexes with immunoreceptors like TREM2 to trigger intracellular signaling pathways. • Phagocytosis: Enhances the ability of immune cells to engulf and digest pathogens. • Cytokine Production: Modulates the production of cytokines, which are essential for immune responses. • Chemotaxis: Aids in the movement of immune cells towards sites of infection or inflammation. • Bone Remodeling: In osteoclasts, DAP12 is involved in bone resorption processes.
Interaction: • TREM2: DAP12 forms a complex with TREM2, which is essential for microglial activation and phagocytosis. • RER1: DAP12 interacts with RER1, which retains unassembled DAP12 in the early secretory compartments, preventing its transport to the plasma membrane. • NKG2D: This receptor on NK cells and some T cells binds to stress-induced ligands, playing a role in immune responses against tumors and infections. • SIRP-1β: Another receptor that pairs with DAP12, contributing to immune cell signaling. • SYK: Spleen tyrosine kinase (SYK) is involved in downstream signaling pathways activated by DAP12. • PI3K: Phosphoinositide 3-kinase (PI3K) is part of the signaling cascade triggered by DAP12.
Ligand/Receptor: • TREM2: Triggering Receptor Expressed on Myeloid cells 2 (TREM2) binds to DAP12, playing a crucial role in microglial activation and phagocytosis. • NKG2D: This receptor on natural killer (NK) cells and some T cells binds to stress-induced ligands such as MICA, MICB, and UL16-binding proteins, which are involved in immune responses against tumors and infections. • SIRP-1β: Signal Regulatory Protein 1β is another receptor that pairs with DAP12, contributing to immune cell signaling
Cell Type: Antigen-presenting cells, Dendritic cells, Macrophages, Monocytes, NK cells, Osteoclasts
Biology Area: Adaptive Immunity, Cell Biology, Immuno-Oncology, Immunology, Innate Immunity, Neuroinflammation, Signal Transduction
Molecular Family: Adaptor Proteins, Innate Immune Signaling
Antigen References: Sjolin H, et al. 2006. J Immunol. 177:2908. Konishi H, et al. 2018. Front Cell Neurosci. 12:1. Liu Y, et al. 2024. Cell Mol Life Sci. 81:302. Liu G, et al. 2023. J Integr Neurosci.22:150.
Regulation: • Protein Interactions: DAP12 interacts with proteins like RER1, which retains unassembled DAP12 in early secretory compartments, preventing its transport to the plasma membrane. • Gene Expression: The expression of DAP12 can be regulated at the transcriptional level by various cytokines and signaling molecules. • Post-Translational Modifications: Phosphorylation of DAP12 can modulate its activity and interactions with other proteins. • Internalization: DAP12 can be internalized into endocytic vesicles, which may regulate its availability and function at the cell surface.
Gene ID: 7305
UniProt: View information about DAP12 on UniProt.org
Clone: A23012B
Regulatory Status: RUO
Other Names: TYROBP (TYRO protein tyrosine kinase binding protein), KARAP (Killer Activating Receptor Associated Protein), PLOSL1 (Polycystic Lipomembranous Osteodysplasia with Sclerosing Leukoencephalopathy 1), DNAX-activation protein 12
Isotype: Mouse IgG1, κ