Biolegend, 648804, Ultra-LEAF™ Purified anti-human PDGFRα Antibody, 1mg

The Platelet-derived growth factor (PDGF) family comprises of four different polypeptide chains encoded by four genes. The four PDGF chains assemble into disulfide-bonded dimers via homo- or heterodimerization. Five different dimeric isoforms have been described so far; PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC, and PDGF-DD. PDGF is a major mitogen for fibroblasts, smooth muscle cells, and others. They exert their functions by interacting with two related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). Upon ligand binding, PDGFRα undergoes dimerization and autophosphorylation, resulting in the formation of docking sites for SH2 domain-containing signaling proteins. These recruited molecules include enzymatic effectors (e.g., Src family kinases, phosphatidylinositol 3-kinase, Phospholipase Cy1, rasGAP, tyrosine phosphatase Syp) and adaptor proteins (e.g., GRB-2, Shc, Nck). The SH2 domains of these proteins specifically recognize phosphorylated PDGFRα, mediating the activation of downstream signaling pathways. PDGFRα plays a key role in many diseases through the above signal transduction.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Human PDGFRα
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the effect of Recombinant Human PDGFRα-Fc Chimera (6 μg/mL) in NIH3T3 cells in a dose dependent manner. ND50 range: 1.5 – 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3720509 (BioLegend Cat. No. 648803) AB_3720509 (BioLegend Cat. No. 648804)
Structure: Homodimer
Distribution: Present in a variety of mesenchymal tissues such as the lungs, heart, intestines, and skin.
Function: The binding of its cognate ligands results in the activation of multiple signaling cascades such as MAPK, STAT, and PI3K pathways resulting in stimulation of cell proliferation, regulation of cell migration, and chemotaxis in wound healing.
Interaction: Mesenchymal cells, Oligodendrocyte
Ligand/Receptor: Human PDGFAA, PDGFAB, PDGFBB, PDGFCC and PDGFDD
Cell Type: Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Claesson-Welsh L. 1994. Prog Growth Factor Res. 5:37-54. Boström H, et al. 1996. Cell. 85:863-73. Heldin CH, et al. 1999. Physiol Rev. 79:1283-316. Fredriksson L, et al. 2004. Cytokine Growth Factor Rev. 15:197-204. Andrae J, et al. 2008. Genes Dev. 22:1276-312. Yao L, et al. 2022. Cell Rep. 40:111192. Vantler M, et al. 2006. FEBS Lett. 580:6769-76. Vassbotn FS, et al. 1994. J Biol Chem.269:13874-9. Li R, et al. 2018. Elife.7:e36865.
Gene ID: 5156
UniProt: View information about PDGFRalpha on UniProt.org
Clone: W22075L
Regulatory Status: RUO
Other Names: Platelet Derived Growth Factor Receptor Alpha, PDGFR2, CD140a, CD140 Antigen-Like Family Member A
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.