Biolegend, 648806, Purified anti-SMC3 Antibody, 100μg

SMC3 (Structural Maintenance of Chromosomes 3) is a core component of the cohesin complex, a group of proteins essential for chromosome cohesion, segregation, DNA repair, and transcriptional regulation in eukaryotic cells. SMC3 belongs to the Structural Maintenance of Chromosomes (SMC) family of ATPases, which includes other members such as SMC1, SMC2, SMC4, SMC5, and SMC6. Together with SMC1A, RAD21 (SCC1), and STAG proteins (SA1/SA2), SMC3 forms the ring-shaped cohesin complex that topologically encircles sister chromatids, holding them together after DNA replication until their separation during mitosis or meiosis. SMC3 and the broader cohesin complex are involved in regulating gene expression through chromatin architecture remodeling. Cohesin interacts with CTCF (CCCTC-binding factor), a key architectural protein, to form chromatin loops and topologically associating domains (TADs). These structures bring enhancers and promoters into close proximity, modulating transcriptional programs necessary for development and differentiation.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein fragment of human SMC3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 2.5 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol
RRID: AB_3717174 (BioLegend Cat. No. 648805) AB_3717174 (BioLegend Cat. No. 648806)
Structure: SMC3 is a 1217 amino acid protein with a predicted molecular weight of 141.5 kD.
Distribution: Located in nucleus
Function: Forms the ring-shaped cohesin complex
Interaction: Forms a heterodimer with SMC1A or SMC1B in cohesin complexes; interacts with RAD21 and STAG proteins
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: Deardorff MA, et al. 2007. Am. J. Hum. Genet. 80:485-94. Beckouët F, et al. 2010. Mol. Cell. 39:689-99. Gil-Rodríguez MC, et al. 2015. Hum. Mutat. 36:454-62. Wang D, et al. 2018. Biomed. Pharmacother.104:788-797.
Gene ID: 9126
UniProt: View information about SMC3 on UniProt.org
Clone: W23212B
Regulatory Status: RUO
Other Names: Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan (Bamacan), BAM, BMH, Chondroitin sulfate proteoglycan 6, CSPG6, Chromosome-associated polypeptide, hCAP
Isotype: Rat IgG2b, κ