Biolegend, 648807, Purified anti-Bcl-XL Antibody, 25μg

Bcl-X, a member of the Bcl-2 family of proteins, is encoded by the BCL2L1 gene and plays a crucial role in regulating apoptosis. Bcl-X exists in two main isoforms: Bcl-XL, which is anti-apoptotic, and Bcl-XS, which is pro-apoptotic. The balance between these isoforms is regulated through alternative splicing, influenced by various splice factors, transcription factors, and signaling pathways. Dysregulation of Bcl-X splicing has been implicated in several diseases, particularly cancer and diabetes. In cancer, overexpression of Bcl-XL can lead to resistance to chemotherapy, while in diabetes, increased Bcl-XS expression can result in apoptosis of pancreatic β-cells.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human Bcl-X protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Due to homology, this antibody may detect the isoform Bcl-XS (18.8 kDa) which is a result of alternative splicing. However, in Western Blot, this isoform was not detected. The canonical sequence, isoform Bcl-XL, was detected by this antibody in Western Blot.
Additional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization either using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803). We DO NOT recommend using True-Phos™ Perm Buffer (Cat. No. 425401). For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using either Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).
RRID: AB_3717173 (BioLegend Cat. No. 648807) AB_3717173 (BioLegend Cat. No. 648808)
Structure: Bcl-XL is a 233 amino acid length protein with a molecular weight of approximately 26 kDa.
Distribution: Mitochondrial membrane and cytoplasm
Interaction: Bcl-XL and Bcl-XS interact with: Bad, Bak, Bim, Bid, Bax and p53
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Death, Cell Proliferation and Viability
Molecular Family: Adaptor Proteins, Mitochondrial Markers, Scaffold Proteins
Antigen References: Dou Z, et al. 2021. J Exp Clin Cancer Res. 40, 194. Bharatham N, et al. 2011. PLOS One. doi.org/10.1371/journal.pone.0026014. Stevens M, et al. 2019. Front Genet.10:doi.org/10.3389/fgene.2019.00804. Findley HW, et al. 1997. Blood. 89:2986.
Gene ID: 598
UniProt: View information about Bcl-XL on UniProt.org
Clone: W23168D
Regulatory Status: RUO
Other Names: BCLX, BCL-X, Bcl-2-like protein 1, BCL2L1, Bcl2-L-1, Apoptosis regulator Bcl-X, BCL2L
Isotype: Rat IgG2a, κ