Biolegend, 648815, Purified anti-Keratin 14 Antibody, 100μg

Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant peptide corresponding to the C terminus of human keratin 14
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, WB, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with any of the following: -Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) -True-Phos™ Perm Buffer (Cat. No. 425401) -True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) -True-Nuclear™ Transcription Factor Buffer Set gives the most robust signal. This product does not cross-react with mouse keratin 14 by Western Blot.
RRID: AB_3717183 (BioLegend Cat. No. 648814) AB_3717183 (BioLegend Cat. No. 648815)
Structure: Keratin 14 is a 472 amino acid protein with a molecular mass of 52 kD.
Distribution: Tissue distribution: skin Cellular distribution: cytoplasm, nucleus
Function: Nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles, enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Interaction: Keratin 5, desmosomal proteins, plectin
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: Wend P, et al. 2013. EMBO J. 32:1977-89. Welm AL, et al. 2005. Proc Natl Acad Sci USA. 102:4324-9 Hu Y, et al. 2001. Nature. 410:710-714. Yuspa SH, et al. 1989. J Cell Biol. 109:1207-1217. Roop DR, et al. 1984. J Biol Chem. 259:8037-8040.
Gene ID: 3861
UniProt: View information about Keratin 14 on UniProt.org
Clone: W24068C
Regulatory Status: RUO
Other Names: Keratin, type I cytoskeletal 14, CK-14, cytokeratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner), KRT, KRT14
Isotype: Rat IgG2b, κ