Biolegend, 648823, Purified anti-FSP1/AIFM2 Antibody, 100μg

FSP1/AIFM2, also known as apoptosis-inducing factor-homologous mitochondrion-associated inducer of death (AMID), functions as an NADH-dependent oxidoreductase and plays a key role in redox homeostasis. It is primarily localized to the outer mitochondrial membrane and the cytoplasm. FSP1/AIFM2 catalyzes electron transfer reactions, which help regulate cellular oxidative stress. Under normal physiological conditions, FSP1/AIFM2 contributes to maintaining cellular redox balance and supports cell viability. When overexpressed or under conditions of metabolic or oxidative stress, FSP1/AIFM2 can trigger caspase-independent apoptosis characterized by chromatin condensation and DNA fragmentation. FSP1/AIFM2 also plays a role in stress-induced cell death and has been proposed to act as a tumor suppressor by inducing cell death in response to oncogenic signals. FSP1/AIFM2 has also been identified as a player in ferroptosis, regulating cell death by iron-dependent lipid peroxidation. FSP1/AIFM2 protects against ferroptosis by acting as a lipid oxidoreductase, reducing lipid peroxides and thereby suppressing ferroptotic cell death. The antioxidant function of FSP1/AIFM2 suggests its dual roles in cell survival, which shifts between promoting and preventing cell death depending on cellular context and environmental stimuli. In neurodegenerative diseases, where oxidative stress and lipid peroxidation are elevated, FSP1/AIFM2’s redox-regulating function positions it as a protective factor under mild oxidative conditions, where it helps neutralize ROS and preserve mitochondrial integrity.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human FSP1/AIFM2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 2.5 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with 100% ice-cold methanol.
RRID: AB_3717208 (BioLegend Cat. No. 648822) AB_3717208 (BioLegend Cat. No. 648823)
Structure: FSP1/AIFM2 is a 373 amino acid protein with a predicted molecular weight of 40.5 kD.
Distribution: Mitochondrion membrane but can also be detected in lipid droplets, cell membrane and cytoplasm.
Interaction: KPNA2, IPO5
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Death, Cell Proliferation and Viability, Mitochondrial Function, Neurodegeneration, Neuroinflammation
Molecular Family: Enzymes and Regulators, Mitochondrial Markers, Tumor Suppressors
Antigen References: Bersuker K, et al. 2019. Nature. 575:688-692. Doll S, et al. 2019. Nature. 575:693-698. Nguyen HP, et al. 2020. Mol. Cell. 77(3):600-617.e4. Dai E, et al. 2020. Biochem. Biophys. Res. Commun. 523:966-971.
Gene ID: 9606
UniProt: View information about FSP1/AIFM2 on UniProt.org
Clone: W23134A
Regulatory Status: RUO
Other Names: AMID, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Ferroptosis suppressor protein 1, PRG3, p53-responsive gene 3 protein
Isotype: Rat IgG2b, κ