Biolegend, 648836, Purified anti-HMGA2 Antibody, 25μg

HMGA2, also known as the high-mobility group AT-hook 2, is a nonhistone transcriptional regulator belonging to the high mobility group (HMG) protein family with AT-hook DNA binding motif. HMGA2 is highly expressed during early mammalian embryogenesis but is hardly detected in normal adult tissues. Recently, it was discovered that HMGA2 is upregulated in a variety of tumors including gastric cancer, ovarian cancer, non-small cell lung cancer and colorectal cancer, suggesting that it has a role in controlling tumor progression. The cancers with overexpression HMGA2 have a significantly stronger response to double strand breaks in DNA caused by radiation therapy and certain chemotherapy treatments. HMGA2 also has been implicated in diet-induced obesity in the gene knockout study of the mouse.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human HMGA2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: The clone cross-reacts with mouse and rat in western blot (WB). For immunocytochemistry (ICC), we recommend 4% PFA fixation (Cat. No. 420801) followed by permeabilization with Triton X-100 or ice-cold methanol. For immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 (Cat. No. 422704) are recommended.
RRID: AB_3720546 (BioLegend Cat. No. 648836) AB_3720546 (BioLegend Cat. No. 648837)
Structure: HMGA2 is a 109 amino acid protein with a predicted molecular weight of 12 kD.
Distribution: Nucleus. Expressed predominantly during embryogenesis.
Function: Transcriptional regulator, cell cycle regulation.
Interaction: E4F1, NEK2
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Stem Cells
Antigen References: Cleynen I, et al. 2008. Int J Oncol. 32:289. Monzen K, et al. 2008. Nat Cell Biol. 10:567. Fusco A and Fedele M. 2007. Nat Rev Cancer. 7:899. Rawlinson N.J, et al. 2008. Cancer Genet Cytogenet.181(2):119. Wei J.J, et al. 2010. Am J Surg Pathol. 34(1):18. Mahajan,A, et al. 2010. Mod Pathol. 23(5),673. Ming- Boo L, et al. 2005. Cancer Res. 65(15):6622. Zhou X, et al. 1995. Nature. 376(6543):771. Anand A, et al. 2000. Nature Genetics. 24:377.
Gene ID: 8091
UniProt: View information about HMGA2 on UniProt.org
Clone: W23012E
Regulatory Status: RUO
Other Names: High Mobility Group AT-Hook 2, High-Mobility Group (Nonhistone Chromosomal) Protein
Isotype: Rat IgG2b, κ