Biolegend, 648855, Ultra-LEAF™ Purified anti-human Thrombopoietin Antibody, 1mg

TPO is a key regulator of megakaryocytopoiesis and thrombopoiesis. TPO binds to its receptor, the cellular homologue of the myeloproliferative leukemia virus oncogene (c-Mpl) and stimulates the proliferation and maturation of megakaryocytes. MPL does not possess kinase activity, thus the receptor associates with intracytoplasmic tyrosine kinases, janus kinase 2 (JAK2), for signal transduction.  JAK2 is also important for MPL stability and cell-surface expression. TPO can promote the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multi-lineage progenitor cells. TPO levels in blood and bone marrow are inversely related to platelet count.  The regulation of TPO levels is mediated by its receptor c-Mpl (uptake and destruction). TPO is upregulated by PDGF and FGF-2, and it is downregulated by PF4, thrombospondin, and TGF-β in bone marrow stromal cells. In hepatocytes, HGF enhances TPO mRNA expression. During acute-phase response, IL-6 induces TPO transcription in the liver. Elevated plasma TPO levels exist in several hematological diseases associated with thrombocytopenia, coronary syndromes, and sepsis. Besides its hematopoietic effects, TPO is expressed in the brain where it promotes apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling. Increased TPO concentrations are present in the cerebrospinal fluid of some patients with bacterial or viral meningitis.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human TPO
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the proliferation induced by recombinant human TPO (Cat. No. 763702) at 1 ng/mL in the MO7e cells. ND50 range: 0.2 - 2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3720579 (BioLegend Cat. No. 648854) AB_3720579 (BioLegend Cat. No. 648855)
Structure: Growth Factor, homodimer
Distribution: TPO is produced by the liver and kidney and is expressed in the central nervous system and bone marrow.
Function: TPO regulates the megakaryocytopoiesis and thrombopoiesis.
Interaction: Megakaryocytes, platelets, hemangioblasts, hematopoietic stem cells, and endothelial cells
Ligand/Receptor: c-Mpl
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Avanzi G, et al. 1988. Br. J. Haematol. 69:359. Deutsch V.R. and Tomer A. 2006. Br. J. Haematol. 134:453. Drachman JG, et al. 1995. J. Biol. Chem. 270:4979. Li J, et al. 1999. Br. J. Haematol. 106:345. Kato T, et al. 1997. Proc. Natl. Acad. Sci. 94:4669. Ehrenreich H, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:862. Royer Y, et al. 2005. J. Biol. Chem. 280:27251. Kaushansky K. 2005. J. Clin. Invest. 115:3339. de Graaf CA and Metcalf D. 2011. Cell Cycle. 10:1582. Zhang J, et al. 2010. J. Interferon Cytokine Res. 7:465. Lupia E, et al. 2012. Mediators Inflamm. 2012:390892. Besancenot R, et al. 2014. Blood. 124:2104.
Gene ID: 7066
UniProt: View information about Thrombopoietin on UniProt.org
Clone: A22080G
Regulatory Status: RUO
Other Names: Megakaryocyte Colony Stimulating Factor (MKCSF), Myeloproliferative leukemia virus
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.