Biolegend, 648861, Ultra-LEAF™ Purified anti-human CD309 (VEGFR2) Antibody, 1mg

VEGF family includes five members, VEGFA (VEGFA165), VEGFB, VEGFC, VEGFD, and placenta growth factor (PlGF). Structurally, these growth factors are homodimers, although heterodimers have been described in complexes that are comprised of VEGFA and PIGS. There are three VEGF receptor tyrosine kinases: VEGFR1 (Flt1), VEGFR2 (Flk1), and VEGFR3 (Flt4). VEGFR1 is expressed on monocytes and macrophages, VEGFR2 on vascular endothelial cells, and VEGFR3 on lymphatic endothelial cells. VEGFR2 also binds proteolytically processed VEGFC and VEGFD. VEGFR2 is the major mediator of the signaling cascades that regulate endothelial cell functions including proliferation, migration and differentiation. Soluble VEGFR2 was initially identified in mouse and human plasma resulting from an endothelial cell surface proteolytic cleavage. VEGFA stimulates ADAM17-dependent shedding of VEGFR2. In addition, its shedding is stimulated by phorbol ester (PMA) and the calcium ionophore, ionomycin. Soluble VEGF1 and VEGFR2 can act as decoy receptors for VEGFA, regulating the availability of the same. In addition, alternative splicing of VEGFR2 produces soluble VEGFR2 that binds VEGFC and subsequently prevents lymph angiogenesis. It has been reported that decreased levels of VEGFR2 in the plasma of preeclamptic women may serve as a marker of endothelial cell dysfunction.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Human VEGFR2-Fc
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by binding of 0.01 μg/mL Biotinylated recombinant human VEGFR2-Fc chimera (Cat. No. 570754) to 1.0 µg/mL immobilized recombinant human VEGF-165 (Cat. No. 583704). ND50 range: 0.08 - 0.5 µg/mL.
Structure: Homodimer
Distribution: Soluble VEGFR2 is derived from endothelial cells
Function: VEGFR2's main function is angiogenesis in physiological and pathological conditions. VEGFR2 transcription is regulated by endogenous VEGFA. Soluble VEGFR2 is a decoy receptor for VEGFA and VEGFC.
Interaction: Endothelial cells
Ligand/Receptor: VEGFA, VEGFC
Cell Type: Endothelial cells
Biology Area: Cancer Biomarkers, Immuno-Oncology, Immunology
Molecular Family: CD Molecules, Fc Receptors, Soluble Receptors
Antigen References: DiSalvo J, et al. 1995. J. Biol. Chem. 270:7717. McColl BK, et al. 2003. J. Exp. Med. 198:863. Albuquerque RJ, et al. 2009. Nat. Med. 15:1023. Guangqi E, et al. 2012. J. Biol. Chem. 287:3029. Koch S, and Claesson-Welsh. 2012. Cold Spring Harb Perspect Med. 2:a006502. Munaut C, et al. 2012. Plos One. 7:e33475. Wehland M, et al. 2013. Int. J. Mol. Sci. 14:9338.
Gene ID: 3791
UniProt: View information about CD309 on UniProt.org
Clone: W21067C
Regulatory Status: RUO
Other Names: Kinase insert domain receptor (KDR), Tyrosin kinase growth factor receptor, CD309 Antigen, VEGFR-2, Fetal liver kinase 1 (Flk1)
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.