Biolegend, 648865, Ultra-LEAF™ Purified anti-human LT-α (TNF-β) Antibody, 1mg

LT-α, also known as TNF-β, is a potent mediator of inflammatory and immune responses. It belongs to the TNF superfamily of ligands and signals through TNFR1 and TNFR2. LT-α is produced by activated T and B lymphocytes, and has similar activities to TNF-α. Like TNF-α, LT-α (TNF-β) is involved in the regulation of various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. LT-α is secreted as a soluble polypeptide, but can form heterotrimers with LT-β, which effectively anchors the LT-α to the cell surface. LT-α is cytotoxic to a wide range of tumor cells. Recombinant human LT-α is secreted 171 amino acid protein (18.6 kD) which forms an active non-disulfide linked homotrimer structure in solution.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Human LT-α (TNF-β)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralization of the cytotoxic effect induced by recombinant human LT-α (TNF-β) (Cat. No. 562603) at 0.35 ng/mL on L929 cells. ND50 range: 0.015 - 0.1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3720597 (BioLegend Cat. No. 648864) AB_3720597 (BioLegend Cat. No. 648865)
Structure: TNF superfamily; trimer
Distribution: Lymphocytes
Function: TNF-b is anti-inflammatory factor, regulates APC, NK, macrophages, production and function of T effector cells. In tumors, induces inhibition of proliferation and apoptosis of endothelial cell.
Interaction: APC, NK, macrophages and T effector cells, epithelial cells
Ligand/Receptor: TNFR1 or TNFR2, and HVEM
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: Fitzgerald K, et al. Eds. 2001. The Cytokine Facts Book. Academic Press San Diego. Aggarwal B, et al. Eds. 1992. Tumor necrosis factors: structure function and mechanism of action. Marcel Dekker Inc. Bonavida B, et al. Eds. 1990. Tumor necrosis factor: structure mechanisms of action role in disease and therapy. Karger Basel. Paul N, et al. 1987. Annu. Rev. Immunol. 6:407. Zhi-Wei, et al. 2021. Front. Immunol. 12: 810285.
Gene ID: 4049
UniProt: View information about LT-alpha on UniProt.org
Clone: W19074A
Regulatory Status: RUO
Other Names: Lymphotoxin-alpha (LT-α), Tumor necrosis factor-β, TNFSF1
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.