Biolegend, 648869, Ultra-LEAF™ Purified anti-mouse CD119 (IFN-γ R α chain) Antibody, 1mg

CDw119 is a 90-100 kD type I transmembrane protein, also known as IFN-γ R α chain or IFN-γRI. The IFN-γ receptor is a complex of a high affinity IFN-γ-binding chain (aka, IFN-γ R α chain) and a second accessory protein required for signal transduction known as IFN-γ R β chain. The IFN-γ R α chain is a member of the class II cytokine receptor family. Binding of IFN-γ induces receptor dimerization and internalization. Signal transduction involves Jak1 and Jak2 protein kinases and involves STAT1 activation. The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
1mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Mouse CD119
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by inhibiting the cytotoxic effect induced by recombinant mouse IFN-γ (Cat. No. 575302) at 0.01 μg/mL in Raw264.7 cells. ND50 range: 0.002 - 0.02 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Structure: Ig superfamily, class II cytokine receptor family, associates with IFN-γ receptor β subunit, 90-100 kD
Distribution: Macrophages, monocytes, B, T, and NK cells, neutrophils, endothelial and epithelial cells, fibroblasts
Function: Activates Jak1, Jak2, STAT1, host defense
Ligand/Receptor: IFN-γ
Cell Type: B cells, Endothelial cells, Epithelial cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Calderon J, et al. 1988. P. Natl. Acad. Sci. USA. 85:4837. Basler C, et al. 2002. Int. Rev. Immunol. 21:305. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.
Gene ID: 3459
UniProt: View information about CD119 on UniProt.org
Clone: W20017H
Regulatory Status: RUO
Other Names: IFN-γR, IFN-γRα, IFN-gamma R1, CDw119
Isotype: Rat IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.