Biolegend, 648870, Purified anti-Fibronectin Antibody, 25μg

Fibronectin is a glycoprotein that plays a critical role in cell adhesion, growth, migration, and differentiation. It is a major component of the extracellular matrix (ECM), where it helps to organize other matrix proteins and anchor cells via integrin receptors. Fibronectin is essential during embryonic development and wound healing, facilitating tissue morphogenesis and repair by guiding fibroblast and endothelial cell movement. It also contributes to mechanotransduction and regulates cellular signaling pathways involved in survival and proliferation. Dysregulation of fibronectin expression or structure has been implicated in fibrosis, cancer metastasis, and chronic inflammation.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human plasma fibronectin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with any of the following: -Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) -True-Phos™ Perm Buffer (Cat. No. 425401) -True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401)
RRID: AB_3720599 (BioLegend Cat. No. 648870) AB_3720599 (BioLegend Cat. No. 648871)
Structure: Fibronectin is a 2477 amino acid protein with a predicted molecular weight of 272.3 kD.
Distribution: Secreted, extracellular space
Function: Adhesion molecule
Interaction: Collagen, Fibrin, heparin domains, Tenascin-C, Laminin, degraded by matrix metalloproteinases
Ligand/Receptor: Integrin receptors, syndecans
Cell Type: Fibroblasts, Mesenchymal cells
Biology Area: Angiogenesis, Cell Adhesion
Molecular Family: Adhesion Molecules
Antigen References: Singh P, et al. 2010. Ann Rev Cell Dev. Biol. 26:397-419. Patten J, Wang K. 2021. Adv Drug Deliv Rev. 70:353-368. Sun Y, et al. 2025. J Cell Sci. 138(6):jcs263834.
Gene ID: 2335
UniProt: View information about FN-1 on UniProt.org
Clone: C6F10
Regulatory Status: RUO
Other Names: FN1, FN
Isotype: Mouse IgG1, κ