Biolegend, 648873, Purified anti-FABP2 Antibody, 100μg

Fatty acid-binding protein 2 (FABP2), also known as intestinal fatty acid-binding protein (I-FABP), is a small intracellular lipid-binding protein primarily expressed in the enterocytes of the small intestine. FABP2 belongs to the family of fatty acid-binding proteins that play crucial roles in lipid metabolism by binding long-chain fatty acids and other hydrophobic ligands. FABP2 is involved in the uptake, transport, and intracellular trafficking of dietary fatty acids from the intestinal lumen to metabolic or storage pathways, acting as a lipid chaperone. It contributes significantly to the regulation of lipid absorption and energy homeostasis. A polymorphism in FABP2, Ala54Thr (rs1799883), results in an amino acid substitution at position 54 and has been extensively studied for its metabolic implications. This variant is linked to several metabolic disorders, including insulin resistance, type 2 diabetes mellitus (T2DM), dyslipidemia, and obesity. In cardiovascular disease, FABP2 variants and expression levels are associated with altered lipid profiles and atherogenic risk, likely due to disrupted lipid transport from the gut to the circulation.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human FABP2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).
RRID: AB_3720601 (BioLegend Cat. No. 648872) AB_3720601 (BioLegend Cat. No. 648873)
Structure: FABP2 is a 132 amino acid protein with a predicted molecular weight of 15.2 kD.
Distribution: Highly expressed in small intestine, with the highest expression levels in the jejunum. Subcellular localization is in the cytoplasm.
Biology Area: Cardiovascular Biology
Molecular Family: Apolipoproteins
Antigen References: Albala C, et al. 2004. Obes. Res. 12:340-5. Tsai IT, et al. 2020. Int J Med. Sci. 17:2338-2345. Sekimori T, et al. 2024. J Integr Neurosci. 23:44.
Gene ID: 2169
UniProt: View information about FABP2 on UniProt.org
Clone: W24079A
Regulatory Status: RUO
Other Names: Fatty acid-binding protein 2, Fatty acid-binding protein, intestinal, FABPI, I-FABP
Isotype: Rat IgG2b, λ