Biolegend, 648882, Purified anti-CD19 Antibody, 25μg

CD19 represents one of the most clinically significant and reliable biomarkers in hematologic medicine. This transmembrane glycoprotein serves as the gold standard for B cell identification and characterization throughout the entire B cell lifecycle—from early development through mature B cell stages. CD19 is a transmembrane glycoprotein that plays a central role in B cell development, activation, and signaling. It functions as a coreceptor that amplifies signaling through the B cell receptor (BCR), lowering the threshold for B cell activation. CD19 forms a complex with other surface molecules such as CD21 and CD8, enhancing calcium signaling and downstream kinase activation. It is expressed from early B cell development through mature B cells but is downregulated upon terminal differentiation into plasma cells. Because of its consistent expression on B cells and role in immune regulation, CD19 is a key target in B cell-directed immunotherapies.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human CD19
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704).
RRID: AB_3720624 (BioLegend Cat. No. 648882) AB_3720624 (BioLegend Cat. No. 648883)
Structure: CD19 is a 556 amino acid protein with a predicted molecular weight of 61.1 kD.
Distribution: Cell surface of B lineage cells (except plasma cells) and follicular dendritic cells
Function: Co-receptor
Interaction: Forms the classic B-cell coreceptor complex with CD21 and CD81, which associates with the core B cell receptor. Also associates with CD9 and CD82, and various intracellular signaling and adaptor proteins such as Lyn, PI3K, Vav1, and BLNK.
Cell Type: B cells, Dendritic cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Bradbury L, et al. 1993. J. Immunol. 151:2915.
Gene ID: 930
UniProt: View information about CD19 on UniProt.org
Clone: W24107D
Regulatory Status: RUO
Other Names: B-lymphocyte antigen CD19, T-cell surface antigen Leu-12, Cluster of Differentiation 19
Isotype: Rat IgG2a, κ