Biolegend, 648894, Ultra-LEAF™ Purified anti-human CD200 Antibody, 100μg

CD200 (OX-2) is a 45 kD cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF). CD200 is composed of 202 amino acid extracellular domain containing two IgSF domains, 27 amino acid transmembrane segment, and a 19 amino acid cytoplasmic domain. CD200 is the only known ligand of the CD200R family, and it is highly conserved in the CNS, expressed mainly by neurons and vascular endothelium.  It is also present in most peripheral cell types, thymocytes, T and B cells, and dendritic cells. CD200 and CD200R interact with each other through N-terminal IgSF domains. Their interaction plays important roles in negatively regulating immune responses and attenuating autoimmune diseases and excessive inflammatory responses against pathogens. High expression of CD200 in CNS is thought to be a mechanism of constitutive immune suppression and is developmentally regulated in the mouse brain. Deficiency in neuronal CD200 may explain the chronic inflammation in human neurodegenerative diseases such as Alzheimer, Parkinson, and multiple sclerosis. CD200 and CD200R interaction has also been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in several malignancies, including CLL, as well as on cancer stem cells.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Immunogen: Recombinant Human CD200
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.009 µg/mL of biotinylated recombinant human CD200-Fc chimera (Cat. No. 770202) to 0.5 µg/mL of immobilized recombinant human CD200R1 (Cat. No. 770104). ND50 range: 0.005 - 0.05 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Structure: Disulfide-linked homodimer
Distribution: Neurons and vascular endothelium in CNS, and most peripheral cell types, thymocytes, T and B cells, and dendritic cells.
Function: Co-stimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
Interaction: Mast cells, basophils, macrophages, and dendritic cells
Ligand/Receptor: CD200R
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Soluble Receptors
Antigen References: Hernangomez M, C. et al. 2014. Curr Pharm Des. 20:4707-22. Walker DG, Lue LF. 2013. Future Neurol. 10.2217/fnl.13.14. Wright GJ, et al. 2003. J Immunol. 171:3034-46. Vaine CA and Soberman RJ. 2014. Adv Immunol. 121:191-211. Minas K, et al. 2006. Crit Rev Immunol. 26:213-30. Wang XJ, et al. 2007. J Neuroimmune Pharmacol. 2:259-64. Wong KK, et al. 2010. J Leukoc Biol. 88:361-72.
Gene ID: 4345
UniProt: View information about CD200 on UniProt.org
Clone: A18042A
Regulatory Status: RUO
Other Names: OX-2 membrane glycoprotein, OX-2, OX2, MRC OX-2, MOX1, MOX2, My033
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.