Biolegend, 649204, Direct-Blot™ HRP anti-GAPDH Antibody, 25microl

This mouse monoclonal GAPDH antibody recognizes human GAPDH, also known as glyceraldehyde-3-phosphate dehydrogenase. GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases. The FF26A/F9 monoclonal antibody has been shown to be useful for Western blotting.
25microl
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD4 lymphocytes
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: The optimal concentration should be determined by titration for each individual assay of interest. Clone FF26A/F9 is only reactive to human GAPDH, and not cross-reactive to mouse or rat GAPDH.
Application References(PubMed link indicates BioLegend citation): Maestre L, et al. 2009. Haematologica. 94:419. Zou L, et al. 2012. J Biol. Chem. 287:7190. PubMed Chen CY, et al. 2012. PNAS. 110:630 PubMed Trifari S, et al. 2013. PNAS. PubMed Liu CC, et al. 2013. Mol. Cell Biol. 33:4334. PubMed Trifari S, et al. 2013. PNAS. 110:18608. PubMed Butin-Israeli V, et al. 2015. Mol. Cell Biol. 35:884. PubMed
Product Citations: Jiang D, et al. 2023. Nat Commun. 14:3642. PubMed Ito M, et al. 2024. PLoS One. e0308330:19. PubMed Gholam MF, et al. 2023. Int J Mol Sci. 24: . PubMed Kyle Cottrell, Sergej Djuranovic 2016. PLoS One. 11:e0167877. PubMed Wang Z, et al. 2019. Sci Rep. 9:1853. PubMed Powell G, et al. 2021. Mol Ther Nucleic Acids. 26:865. PubMed Zu T, et al. 2021. Lab Invest. 101:636. PubMed
RRID: AB_2734517 (BioLegend Cat. No. 649204) AB_2566698 (BioLegend Cat. No. 649203)
Structure: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution: Ubiquitously expressed, locate in cytoplasm and perinuclear region.
Function: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and and nicotinamide adenine dinucleotide (NAD). Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretion pathway.
Interaction: Interacts with TPPP. Interacts with EIF1ADand WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Signal Transduction, Western Blot Controls
Antigen References: 1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335. 2. Meyer-Siegler K, et al. 1991. P. Natl. Acad. Sci. USA 88:8460. 3. Hara MR and Snyder SH. 2006. Cell Mol. Neurobiol. 26:527. 4. Zheng L, et al. 2003. Cell. 114:255. 5. Wang Q, et al. 2005. FASEB J. 19:869. 6. Bae BI, et al. 2006. P. Natl. Acad. Sci. USA. 103:3405.
Gene ID: 5590
UniProt: View information about GAPDH on UniProt.org
Clone: FF26A/F9
Regulatory Status: RUO
Other Names: glyceraldehyde-3-phosphate dehydrogenase
Isotype: Mouse IgG1, κ