Biolegend, 650053, Alexa Fluor® 594 Anti-Claudin 6 Antibody, 25μg

Claudin-6 is a member of the claudin family of proteins, which consists of at least 24 members. Claudin proteins are integral membrane proteins characterized by 4 transmembrane regions and 2 extracellular loops that form tight junctions. Abnormal expression can disrupt tight junction integrity, playing an important role in the occurrence and development of cancers. Claudin-6 in particular, has been considered a therapeutic target because it is commonly expressed in tumor tissue but not in healthy adult tissue.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human Claudin-6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Application Notes: For use of this antibody in immunohistochemistry on formalin-fixed paraffin embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704)
RRID: AB_3683487 (BioLegend Cat. No. 650053) AB_3683487 (BioLegend Cat. No. 650054)
Structure: Consists of four transmembrane domains and two extracellular loops. It is a 220 amino acid length protein with a predicted molecular weight of 23 kDa.
Distribution: Cellular membrane
Function: Tight junctions and cell adhesion
Antigen References: Reinhard K, et al. 2020. Science. 367(6476):446-453. Huan D, et al. 2021. Mol Med Rep. 24(3):677. Ushiku T, et al. 2012. Histophatology. 61(6):1043-56. Gowrikumar S, et al. 2019. Int J Mol Sci. 21:53.
Gene ID: 9074
UniProt: View information about Claudin-6 on UniProt.org
Clone: W22275B
Regulatory Status: RUO
Other Names: Claudin-6, Claudin 6, CLDN6, Skullin
Isotype: Rat IgG2a, λ