Biolegend, 650601, Purified anti-Bcl11b Antibody, 25μg

The transcription factor Bcl11b is expressed in thymus, mainly T cells. It is indispensable for T lineage development. When Bcl11b was deleted, T cells from all developmental stages acquired NK cell properties and concomitantly lost or decreased T cell-associated gene expression. These induced T-to-natural killer (ITNK) cells, which were morphologically and genetically similar to conventional NK cells, killed tumor cells in vitro , and effectively prevented tumor metastasis in vivo . Therefore, ITNKs may represent a new cell source for cell-based therapies.
25μg
Verified Reactivity: Human
Reported Reactivity: Mouse, Zebrafish
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human Bcl11b
Formulation: This antibody is provided in Hepes-buffered solution, pH 7.5, 0.02% NaN3.
Preparation: Protein G affinity purified.
Concentration: 0.5 mg/mL
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB, ICC - Quality tested CHIP, IHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting and immunofluorescence microscopy. Western blotting, suggested working dilution(s): Use 0.5 µg antibody per ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution of 1:200. The optimal concentration should be determined by titration for each individual assay of interest.
Application References(PubMed link indicates BioLegend citation): Tomassy GS, et al. 2010. P. Natl. Acad. Sci. USA 107:3576. Cherrier T, et al. 2009. Oncogene 28:3380. Kosulin K, et al. 2013. PLoS One. 8:63646. PubMed.
Product Citations: Tian JL, et al. 2023. Cells. 12:. PubMed Lee SW, et al. 2015. Sci Rep. 16523:5. PubMed Lo JH, et al. 2018. Mol Cancer Ther. 2377:17. PubMed Barford K et al. 2017. The Journal of comparative neurology. 525(8):1861-1878 . PubMed Bott CJ et al. 2019. Mol Biol Cell. 30(10):1214-1229 . PubMed Kosulin K, et al. 2013. PLoS One. 8:63646. PubMed
RRID: AB_10896795 (BioLegend Cat. No. 650601) AB_10915967 (BioLegend Cat. No. 650602)
Structure: 894 amino acids with molecular weight of 96 kD. It encodes a C2H2-type zinc finger protein and is closely related to BCL11A.
Distribution: Nucleus
Function: Tumor-suppressor protein involved in T-cell lymphomas. A key regulator of both differentiation and survival during thymocyte development.
Interaction: Interacts with TFCOUP1, SIRT1, ARP1, and EAR2
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Liu P, et al. 2011. Immunol. Rev. 238:138. 2. Ikawa T, et al. 2010. Science 329:93. 3. Li L, et al. 2010. Science 329:89. 4. Li P, et al. 2010. Science 329:85. 5. Tydell CC, et al. 2007. J. Immunol. 179:421. 6. Avram D, et al. 2002. Biochem. J. 368:555.
Gene ID: 64919
UniProt: View information about Bcl11b on UniProt.org
Clone: 25B6
Regulatory Status: RUO
Other Names: B-cell CLL/lymphoma 11B (zinc finger protein), CTIP2, B-cell lymphoma/leukemia 11B, CTIP-2, hRIT1-alpha, SMARCM2
Isotype: Rat IgG2a