Biolegend, 650702, Purified anti-Galectin-9 Antibody, 100μg

Galectin-9 is a mammalian lectin with a molecular weight around 40 kD. It is a member of the β-galactoside-binding family. With two conserved carbohydrate recognition domains (CRDs), galectin-9 binds small β-galactosides as well as complex glycoconjugates.  HAVCR2/TIM3 has been reported as one of its ligands. Galectin-9 may be retained intracellularly or transported to the cell surface where it can be cleaved to generate a soluble form. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts, and thymus epithelial cells. It can be induced by cytokines in various cell types and is involved in cell aggregation, adhesion, chemotaxis, and apoptosis; galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Galectin-9 (S-type)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, 0.09% NaN3.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Does not cross react with mouse.
Application References(PubMed link indicates BioLegend citation): Pielage JF, et al. 2007. Int. J. Biochem. Cell. Biol. 39:1886. (WB)
RRID: AB_10900999 (BioLegend Cat. No. 650701) AB_10897952 (BioLegend Cat. No. 650702)
Structure: 355 amino acids; 40 kD
Distribution: Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells.
Function: Cell aggregation, adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, and induction of regulatory T cells.
Ligand/Receptor: HAVCR2/TIM3
Cell Type: Lymphocytes, Dendritic cells, Neutrophils, Eosinophils, Astrocytes, Endothelial cells, Fibroblasts, Epithelial cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Antigen References: 1. Seki M, et al. 2008. Clin. Immunol. 127:78. 2. Tsubo IY, et al. 2007. Clin. Immunol. 124:221. 3. Zhu C, et al. 2005. Nat. Immunol. 6:1245. 4. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.
Gene ID: 3965
UniProt: View information about Galectin-9 on UniProt.org
Clone: 9S2-1
Regulatory Status: RUO
Other Names: Lgals9, Gal-9, Ecalectin, Tumor antigen HOM-HD-21, Ecalectin
Isotype: Mouse IgG1, κ