Biolegend, 651002, Purified anti-STAT3 Phospho (Tyr705) Antibody, 100μg

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH conjugated modified synthetic peptide
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ChIP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:500 - 1:1000 dilution). For immunocytochemistry, a concentration of 0.5 μg/ml is recommended. For ChIP applications, the suggested dilution is 1:50 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting. During PD testing, this clone failed to stain PFA-fixed cells that had been permeabilized with Triton X-100. We highly recommend permeabilizing cells with methanol.
Product Citations: Jafari S, et al. 2023. Bioimpacts. 13:51. PubMed Correa-Araujo L, et al. 2023. Stem Cell Res Ther. 306:14. PubMed Wang W, et al. 2018. Cancer Cell. 34:757. PubMed Jafari S, et al. 2020. Daru. 28:159. PubMed Chen HL, et al. 2021. Pharm Biol. 59:134. PubMed De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
RRID: AB_10897947 (BioLegend Cat. No. 651001) AB_10900983 (BioLegend Cat. No. 651002)
Structure: STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution: Ubiquitous.
Function: STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.
Cell Sources: Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.
Cell Type: Embryonic Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: 1. Akira S, et al. 1994. Cell 77:63. 2. Zhang X, et al. 1995. Science 267:1990. 3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30. 4. Simon A, et al. 2000. Science 290:144. 5. Hoey T, et al. 1999. Adv. Immunol. 71:145.
Regulation: The small GTPase Rac1 binds and regulates activity of STAT3.
Gene ID: 6774
UniProt: View information about STAT3 Phospho Tyr705 on UniProt.org
Clone: 13A3-1
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
Isotype: Mouse IgG1, κ