Biolegend, 651022, PerCP/Cyanine5.5 anti-STAT3 Phospho (Tyr705) Antibody, 100tests

Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH conjugated modified synthetic peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.
Product Citations: Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
RRID: AB_2728485 (BioLegend Cat. No. 651021) AB_2728486 (BioLegend Cat. No. 651022)
Structure: STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
Distribution: Ubiquitous.
Function: STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.
Cell Sources: Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.
Cell Type: Embryonic Stem Cells, Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: 1. Akira S, et al. 1994. Cell 77:63. 2. Zhang X, et al. 1995. Science 267:1990. 3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30. 4. Simon A, et al. 2000. Science 290:144. 5. Hoey T, et al. 1999. Adv. Immunol. 71:145.
Regulation: The small GTPase Rac1 binds and regulates activity of STAT3.
Gene ID: 6774
UniProt: View information about STAT3 Phospho Tyr705 on UniProt.org
Clone: 13A3-1
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.