Biolegend, 651201, Purified anti-ATM Phospho (Ser1981) Antibody, 25μg

Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. ATM is held inactive in unirradiated cells as a dimer or higher-order multimer. Cellular irradiation induces rapid intermolecular autophosphorylation of serine 1981 that causes dimer dissociation and initiates cellular ATM kinase activity. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KLH conjugated synthetic peptide SLAFEEGSpQSTTISS
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg per mL antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Patent No. USA: 7,108,992
Product Citations: Benjaskulluecha S, et al. 2024. iScience. 108733:27. PubMed Liu H, et al. 2018. Oncotarget. 3.373611111. PubMed Bhandaru M, et al. 2015. PLoS One. 10: 0134678. PubMed Branigan TB, et al. 2021. Cell Reports. 34(9):108808. PubMed Luo Q, et al. 2018. Cell Physiol Biochem. 51:2421. PubMed
RRID: AB_10900815 (BioLegend Cat. No. 651201) AB_10900251 (BioLegend Cat. No. 651202)
Structure: 3056 amino acids, 350 kD.
Distribution: Primarily nuclear, also found in endocytic vesicles.
Function: Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis, and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family: Phospho-Proteins
Antigen References: 1. Lee JH and Paull TT 2007. Oncogene 26:7741. 2. Bakkenist CJ and Kastan MB 2003. Nature 421:499. 3. McConville CM, et al. 1996. Am. J. Hum. Genet. 59:320. 4. Tang X, et al. 2008. Mol. Cell Biol. 28:2559.
Gene ID: 472
UniProt: View information about ATM on UniProt.org
Clone: 10H11.E12
Regulatory Status: RUO
Other Names: Ataxia telangiectasia mutated (ATM) kinase, serine-protein kinase, AT mutated
Isotype: Mouse IgG1, κ